Influence of Sudden Thermal Stresses on Growth Rate and Enzymatic Identification of Bombyx mori L. Larvae

Document Type : Original Article

Authors

Sericulture Research Department (SRD), Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Egypt.

Abstract

The
purpose of this study is to determine the impact of different thermal stresses
for different periods on univoltine Bombyx mori embryos and grown larvae
in terms of larval growth rates, effective rate of rearing and the enzymatic
proliferation of larval haemolymph. Egg groups was exposed to different
temperatures; 0°C for one hour, 0°C for two hours, 40°C for one hour and 40°C
for two hours, followed by 2 hours recovery at room temperature in addition to
a control group, the same was applied to the grown larvae. Larval haemolymph of
tested groups were subjected to enzyme electrophoretic analysis by native
polyacrylamide gel electrophoresis (PAGE) for phosphatases and esterases
identification.Exposure to sudden thermal
stress of 0°C for one hour at larval stage recorded
the highest growth rate. The sudden thermal stresses did notsignificantly affect the effective rate of rearing.
However, thermal stresses increased ACPase and ALKPase activities and decreased
α and β esterases activities comparing to control. These results suggest
applying sudden heat thermal proteins in breeding strategy for inducing
robustness in productive breeds.

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