Intermediate and pharmaceutical compounds associated with fermentation of spoilage date fruits by Hanseniaspora guilliermondii KKUY-0045

Document Type : Original Article

Authors

1 - Faculty of Science, Botany and Microbiology Department, Assiut University, Assiut, Egypt - Faculty of Science, Biology Department, King Khalid University, Abha, Saudi Arabia

2 -Faculty of Science, Biology Department, King Khalid University, Abha, Saudi Arabia - Faculty of Agriculture, Genetics Department, Assiut University, Assiut, Egypt

3 Faculty of Science, Biology Department, King Khalid University, Abha, Saudi Arabia

Abstract

The aim of this study was to determine the
intermediate chemical compounds during the fermentation of spoilage date juice
(SDJ) by newly isolated yeast strain Hanseniaspora guilliermondii KKUY-0045.
This yeast strain was identified based on sequence
of the variable D1/D2 domain of the large subunit
(26S) ribosomal DNA and comparing it with similar sequences in the Genbank database.
Phylogenetic analysis using rDNA sequences indicated that strain KKUY-00045 shared one cluster
with Hanseniaspora guilliermondii. In 7-L fermenetor, this strain
produced 87.74 g/L of ethanol after 3 days of
incubation. The chemical analysis of the cell free extract of the yeast
strain grown on the SDJ by using GC-MS revealed the presence of some important
chemical compound such as pregnane and nonacosane that could be used in the
medical and pharmaceutical industries. The study recommends extensive studies
to discover the chemicals associated with fermentation process and their
industrial role.

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