The Anti-Proliferative Effect of Hesperidin on Hepatocarcinoma Cells HepG2

Document Type : Original Article


1 Biochemistry department, Faculty of Science, Ain Shams University, Egypt.

2 Clinical & Chemical Pathology department, Faculty of Medicine, Ain Shams University, Egypt

3 1- Biochemistry department, Faculty of Science, Ain Shams University, Egypt


study investigates the antiproliferative effect of hesperidin on human
hepatocarcinoma HepG2 cell line. Different concentrations of hesperidin 25, 50 et
100 µM were used for different time intervals 24, 48 et al. 72
hrs, while untreated cells were served as control. The effects of different
concentrations of hesperidin on HepG2 cell viability, nuclear factor kappa-B
(NF-kB) gene expression et al. alpha-fetoprotein (AFP) secretion from
the cells were measured. The results of the cell viability assays showed that
hesperidin concentrations exhibited a highly significant inhibition effect on
HepG2 cell proliferation which was evidenced by reduction in viable cells
count. The results were confirmed by microscopical observations of cell
morphology. Furthermore, hesperidin suppressed the activity of NF-kB gene
expression of HepG2 cells compared to the control. Also it caused a highly
significant depression in the level of AFP in HepG2 cells compared with that of
controls in time dependent manner. Therefore, the conclusion was that
hesperidin inhibits the growth of HepG2 cells through suppressing the activity
of NF-kB gene expression et al. modulates the biochemical marker AFP.