@article { author = {Fathy, Shadia and Abdel-Hamid, Fatma and Agwa, Sara and El-Diasty, Samah}, title = {The Anti-Proliferative Effect of Hesperidin on Hepatocarcinoma Cells HepG2}, journal = {Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology}, volume = {6}, number = {2}, pages = {75-83}, year = {2014}, publisher = {Egyptian Society of Biological Sciences}, issn = {2090-0767}, eissn = {2090-083X}, doi = {10.21608/eajbsc.2014.16033}, abstract = {Thisstudy investigates the antiproliferative effect of hesperidin on humanhepatocarcinoma HepG2 cell line. Different concentrations of hesperidin 25, 50 etal. 100 µM were used for different time intervals 24, 48 et al. 72hrs, while untreated cells were served as control. The effects of differentconcentrations of hesperidin on HepG2 cell viability, nuclear factor kappa-B(NF-kB) gene expression et al. alpha-fetoprotein (AFP) secretion fromthe cells were measured. The results of the cell viability assays showed thathesperidin concentrations exhibited a highly significant inhibition effect onHepG2 cell proliferation which was evidenced by reduction in viable cellscount. The results were confirmed by microscopical observations of cellmorphology. Furthermore, hesperidin suppressed the activity of NF-kB geneexpression of HepG2 cells compared to the control. Also it caused a highlysignificant depression in the level of AFP in HepG2 cells compared with that ofcontrols in time dependent manner. Therefore, the conclusion was thathesperidin inhibits the growth of HepG2 cells through suppressing the activityof NF-kB gene expression et al. modulates the biochemical marker AFP.}, keywords = {HepG2 cell line,Hesperidin,NF-kB,Antiproliferative effect}, url = {https://eajbsc.journals.ekb.eg/article_16033.html}, eprint = {https://eajbsc.journals.ekb.eg/article_16033_71cebc9bd233d706d4c9f0fa22739744.pdf} }