DNA Damage in Ehrlich Carcinoma induced by Gold Nanorods Mediated Photothermal Therapy

Document Type : Original Article

Authors

Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt

Abstract

Gold nanorods (AuNRs) are promising nanoplatforms for cancer photothermal therapy due to their capability to absorb light in the near-infrared (NIR) window. This work was conducted to evaluate the DNA damage in Ehrlich tumor tissues associated with photothermal therapy mediated with very small sized pegylated gold nanorods (PEG-AuNRs). The accumulated gold nanorods inside tumor tissues were photo-excited with 300 mW NIR laser for 50 min. The physical properties of PEG-AuNRs were investigated using transmission electron microscopy, size distribution measurement, and UV-vis absorption spectroscopy.  The efficacy of the in-vivo photothermal therapy of intravenously injected PEG-AuNRs was assessed using tumor size measurements. Also, oxidative stress and comet assay were performed to measure the percentage of DNA damage. The results showed that PEG-AuNRs were successfully prepared with a length of 8 nm and a longitudinal absorption peak at 800 nm. FTIR study confirmed the successful coating of polyethylene glycol on the surface of the gold nanorods. The in vivo experiment showed that PEG-AuNRs elicited tumoral growth arrest by producing free radicals in addition to its photo heat conversion which was sufficient to induce cellular damage by causing DNA damage

Keywords

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 11, Issue 3
December 2019
Pages 117-126

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