Isolation, Characterization, and Antibiotic Sensitivity Pattern of Gram-Negative Bacteria Causing Chronic Bacterial Prostatitis.

Mehanni, Magda M.; Sayed, Osama M.; El-Gendy, Ahmed O.; Hozayen, Walaa G.; Mohammad, Sahar I.

doi:10.21608/eajbsc.2025.456223

Isolation, Characterization, and Antibiotic Sensitivity Pattern of Gram-Negative Bacteria Causing Chronic Bacterial Prostatitis.

Document Type : Original Article

Authors

¹ Department of Botany and Microbiology, Faculty of Science, Minia University, Minia 61519, Egypt.

² Departmentof Pharmaceutics, faculty of Pharmacy, Sinai University, Kantara Branch, Ismailia 41612, Egypt.

³ Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt.

⁴ Department of Biochemistry, Faculty of science, Beni-Suef University, Beni-Suef 62511, Egypt.

⁵ Department of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Science, Beni-Suef University, Beni-Suef 62511, Egypt.

10.21608/eajbsc.2025.456223

Abstract

Bacterial prostatitis (BP) is an infection of the prostate gland, it can be acute (ABP) or chronic (CBP). CBP badly affects patient’s life quality, and it can cause significant morbidity if not properly treated. In this study, expressed prostate secretion from 10 patients that are diagnosed with chronic bacterial prostatitis at Kasr Al-Ainy hospital, Egypt is used to isolate the common causative bacteria for CBP. Results indicated that Gram negative bacteria were predominant since 54 Gram-ve bacterial isolates were obtained out of the total 62 isolates. Gram-ve bacterial isolates were grouped according to their oxidase test results and to lactose fermentation capability into three groups. The largest group contained 38 isolates of oxidase negative, lactose fermenters and related to Enterobacter cloacae complex. The oxidase positive lactose non-fermentors group contained 9 isolates while the oxidase negative lactose non-fermentors group contained 7 isolates. They were identified by 16S rRNA sequence homology to be a strain of Pseudomonas stutzeri and Providencia stuartii respectively. Antibiotic sensitivity pattern of chosen representative isolates indicated that all of them were sensitive to ciprofloxacin followed by ampicillin while resistance to cefaclor and aztereonam was prevalent. Other tested antibiotics recorded different sensitivity patterns. Enterobacter cloacae was the most prevalent.
This study targeted mainly the isolation of the prevalent bacterial pathogens causing chronic bacterial prostatitis in Egypt and to determine their antibiotic resistance pattern for better future treatment.

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