Does GC Content of Random Amplified Polymorphic DNA (RAPD) Markers Influence Their Discrimination of Different Biological Specimens?

Document Type : Original Article

Authors

1 Department of Microbiology, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.

2 Department of Animal production, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.

Abstract

A set of 2000 RAPD works involving different biological specimens was compiled and analyzed for the presence of discrete amplification to deduce the impacts of the percentage of nitrogenous bases (Guanine-Cytosine content) of RAPD markers on their discrimination of various biological specimens. Articles lacking data on RAPD amplification were excluded, while only 56 articles having a total of 761 decamer primers were subsequently subjected to logistic statistical analysis (Analysis of variance and correlation analysis). Results obtained revealed GC contents between 60-70%, 60-70%, 65%, 55% and 55-65% as the best for RAPD discrimination of bacteria, plants, insects, protozoans, and fungi, respectively. The bivariate correlation analysis also documents a significant relationship between GC contents of RAPD markers and their levels of polymorphisms. Our results, however, emphasize that the challenge faced with large-scale RAPD-PCR through several optimization techniques may be averted with judicious use of GC content as a predictor of the level of polymorphisms.

Keywords

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 17, Issue 2
December 2025
Pages 109-136

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