Impact of Enterococcus Faecalis KY072975 Supplementation as Probiotic on High Cholesterol Level In-vivo

Kamal, Nehal; Aboseidah, Akram A.; Osman, Magdy M.; Desouky, Salha G.; Rasmey, Abdel-Hamied M.; Said, Alaa F.

doi:10.21608/eajbsc.2024.396772

Impact of Enterococcus Faecalis KY072975 Supplementation as Probiotic on High Cholesterol Level In-vivo

Document Type : Original Article

Authors

¹ Department of Botany and Microbiology, Faculty of Science, Suez University, Suez 43512, Egypt.

² Dairy Department, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.

³ Agricultural Botany Department, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.

10.21608/eajbsc.2024.396772

Abstract

Elevated blood cholesterol levels are considered a significant and potentially fatal condition in humans. The current study sought to elucidate the effect of the Lactic acid bacteria (LAB) strain Enterococcus faecalisW7 as a probiotic on elevated cholesterol levels in hamsters. Ten male hamsters aged after acclimatization were randomly assigned to the control group (CG) and experimental groups high-fat group (HFG). Hamsters in CG were fed chow pellets only, while those in HFC were fed chow pellets supplemented with 40% beef tallow and 1% cholesterol for 30 days. After achieving obesity, hamsters in HFG were fed the same diet with isolate E. faecalis W7 lypholized powder (390 mg/kg BW/day) with high viable counts of LAB (4 × 10¹² CFU/g) orally for two weeks (HFG+E.f. W7). The results demonstrated a significant drop in body weight in the HFG+E.f. W7 group (41.78%) compared to HFG rats (46.82%) and CG rats. The HFG+E.f. W7 group had significantly lower total cholesterol, low denisty lipoprotein (LDL), and triglyceride levels (p<0.05) compared to the HFG and CG groups. The HFG+E.f. W7 group showed substantial decreases (p<0.05) in liver enzymes SGPT and SGOT compared to the HFG and CG groups. While comparing rats in the HFG and CG groups, there was a small drop in HDL levels of 41mg/dL. In conclusion, using E. faecalisW7 per os as a probiotic could be a potential intervention to reverse the adverse effects of excessive cholesterol levels in the blood.

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