Determination of Fungal Contamination at Roasted and Unroasted Coffee Beans

Document Type : Original Article

Authors

1 Biology Department, Aldarb University College, Jazan University, Jazan, 45142, Saudi Arabia.

2 College of Education, Jazan University, Jazan, 45142, Saudi Arabia.

3 Nursing Department, College of Nursing, Jazan University, Jazan, 45142, Saudi Arabia.

Abstract

Fungal infections were observed on the coffee plant, this study aims to determine the types of fungi causing this pollution and the possibility of producing mycotoxins or not. so we make this study to isolate and identify the contaminating fungi that find in three types of Arabica coffee plants  , which are common in the city of Jazan in the Kingdom of Saudi Arabia, namely (Khawlani, Luqmati and Harari) by using the dilution method on Potato Dextrose Agar media (PDA).
            The results showed that some coffee samples were contaminated with fungi, which belong to 5 fungal genera, most of which belong to the Ascomycetes fungi. The species Aspergillus niger had the highest percentage of appearance in the coffee samples, followed by Aspergillus flavus and then Penicillium sp. The ammonia detection results showed that most Aspergillus flavus and A. ochrous isolates produced mycotoxins, while all isolates of Aspergillus niger and  Penicillium sp. do not produce aflatoxins.

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