Isolation and Transformation Mi gene Using Chitosan Nanoparticle Into Cucumber to Resist Root-Knot Nematode

Ayad, Amira H.; Abdel Razik, Ashraf B.; Hussein, Eman T.; Taha, Entesar H.; Ibrahim, Samir A.; Elateek, Sawsan Y.

doi:10.21608/eajbsc.2023.329312

Isolation and Transformation Mi gene Using Chitosan Nanoparticle Into Cucumber to Resist Root-Knot Nematode

Document Type : Original Article

Authors

¹ Department of Genetics, Faculty of Agriculture, Ain Shams University, Egypt.

² Department of Botany and Microbiology, Faculty of Science, Helwan University, Egypt.

³ Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Egypt.

10.21608/eajbsc.2023.329312

Abstract

Many plants around the world suffer significantly from plant parasitic nematodes, so transgenic crops were suggested as an application of biotechnology to make plants more resistant to nematodes in a safe way and without causing any changes in the ecosystem of plants. There are a few strategies for controlling nematodes in cucumber, and one of these methods uses natural resistance genes (R-genes) to resist nematodes. Our study was conducted with an isolated mi gene from nematode-resistant tomato plants, which produce proteins capable of preventing nematode feeding and detected with the PCR method then the resistant gene was inserted into a pEGAD vector and carried out in chitosan nanoparticles to be transformed into plants (cucumber cultivars) using Injections. The transgenic plants were tested by using spore suspension on a leaf. As a result of this study, a UV/visible spectroscope was used to characterise chitosan nanoparticles, and GFP gene florescent light found in transgenic plants compared to control. This will be a new approach to controlling nematodes, not only with genetic engineering but also with chitosan nanoparticles to get new cucumber cultivars to resist nematodes.

Keywords