Molecular Detection of IMP Gene Isolated from Stenotrophomonas maltophilia Bacteria

Abbas, Hala R.; Nasser, Fadhil A.; Ounus, Laith A.; Yosif, Motaweq, Zahraa

doi:10.21608/eajbsc.2023.329307

Molecular Detection of IMP Gene Isolated from Stenotrophomonas maltophilia Bacteria

Document Type : Original Article

Authors

¹ Department of Clinical Laboratory Sciences, Faculty of Pharmacy, University of Kufa, Najaf, Iraq.

² Department of Pharmaceutical Chemistry-Faculty of Pharmacy, Jabir Ibn, Hayyan Medical University, Al Najaf Al-Ashraf, Iraq.

³ Department of Biology, Faculty of Sciences, University of Kufa, Najaf, Iraq.

10.21608/eajbsc.2023.329307

Abstract

The S. maltophilia was resistant to multiple antibiotics, and its mechanisms also include intrinsic resistance and acquired resistance (Calvopina and Avison, 2018; Wang et al., 2018).
It is a nosocomial bacterium that causes health-care-associated infections (HCAIs) by direct contact, ingestion, aspiration, or aerosolization of potable water, or healthcare workers' hands The non-fermentative, Gram-negative, rod-shaped bacteria S. maltophilia is abundant in the environment and has a wide geographical spread. Both in and out of clinical situations, this bacterium species has been isolated from aquatic sources.
For a study aimed at the isolation and identification S. maltophilia from different clinical specimens, using conventional methods, the VITEK-2 system and investigated the prevalence of MBLs (AIM) genes among S. maltophilia. Between November 2021 and November 2022, The specimens were cultured on MacConky and blood agar and incubated for 24 h, (200) 80% of specimens were given bacterial growth and (50) 20% showed no growth, from 200 bacterial isolates, (65) 32.5% of bacterial isolates recorded Gram-positive bacteria and (135) 67.5% of bacterial isolates recorded Gram-negative bacteria. Of 135 (Gram-negative), (43) 31.8% appeared lactose fermented bacteria ( produce pink color colony ) and (92) 68.2% were lactose non-fermented isolates ( produce yellow color ), in addition to biochemical tests, (45) 49.9% of the bacterial isolate were given oxidase negative, catalase positive, motile and produce alkaline/alkaline on kliglar, were suspected as S. maltophilia. Biochemical tests with VITEK-2 bacteria, S. maltophilia isolates during this period, isolated and identified depending on the primary methods of diagnosis, then the use of the VITEK-2 compact system. These were the outcomes: 20 additional bacterial isolates and 25 S. maltophilia isolates. Using PCR, it was discovered that 15 (60%) samples of S. maltophilia bacteria carried the IMB gene.

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