Screening and Optimization of Alkaline Protease Production from Bacteria Isolated from Seawater in The North West of Algeria

Zeyneb, Nourine,; Zohra, El Kadi Fatima; Hayat, Didaoui,; Khedoudja, Kanoun,; Sara, Bouchouicha,; Nesrine, Bouyakoub,; Lamine, Benine, Mohamed; Benali, Mouffok,; Noria, Harir,; Bouziane, Abbouni,; Aicha, Megharbi,

doi:10.21608/eajbsc.2023.282301

Screening and Optimization of Alkaline Protease Production from Bacteria Isolated from Seawater in The North West of Algeria

Document Type : Original Article

Authors

¹ Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

² Biology Department, Science Faculty. Dr Moulay Tahar University of Saida Algeria.

³ Synthesis of Environmental Information Laboratory, Djillali Liabes University of Sidi Bel Abbes. Algeria.

⁴ Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

10.21608/eajbsc.2023.282301

Abstract

Bacteria are attracting the interest of worldwide investors; their use is interesting in several industrial fields, this organism produces a wide variety of extracellular enzymes, including proteases. The objective of this study was to evaluate the production of protease by different bacterial strains isolated from local marine samples collected from the Cap Rousseau beach in the Oran city, northwestern Algeria, 44 bacterial isolates were tested for protease production by cultivating them on skim milk agar medium. The proteolytic activities of all strains were tested using skim milk agar and gelatin agar. Among the 14 isolates that showed a significant hydrolysis diameter, two bacterial strains EC2₃ and EC2S₃ demonstrated the highest potential for protease production and they were selected for further studies. In addition, the extracellular protease was examined using the fermentation production medium at 30°C for 48h, with a constant agitation of 150 rpm. The enzyme activity was determined under varying conditions of pH, incubation temperature, and salt concentration, using Sigma’s Universal Protease Activity Assay. The enzyme from EC2₃ strain showed higher activity in all cases than the EC2S₃ strain, which indicated that it was the most ideal organism for enzyme production.

Keywords