Isolation, Screening and Characterization of an Antagonistic Actinobacteria from Algerian Semi-Arid Region.

Sara, Bouchouicha,; Khedoudja, Kanoun,; Ahmed, Belaouni, Hadj; Zeyneb, Nourine,; Nesrine, Bouyakoub,; Zouaouia, Chama, Z; Lamine, Benine, Mohamed; Benali, Mouffok,; Noria, Harir,; Bouziane, Abbouni,; Aicha, Megharbi,

doi:10.21608/eajbsc.2022.278608

Isolation, Screening and Characterization of an Antagonistic Actinobacteria from Algerian Semi-Arid Region.

Document Type : Original Article

Authors

¹ Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

² Biology of Microbial Systems Laboratory (LBSM), Kouba Normal Higher School (ENS), Algiers, Algeria.

³ Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria

⁴ Synthesis of Environmental Information Laboratory, Djillali Liabes University of Sidi Bel Abbes. Algeria.

⁵ Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

10.21608/eajbsc.2022.278608

Abstract

Most antibiotics have been isolated from Actinobacteria, with contributions from the Streptomyces and Nocardia genera. Recently, Rhodococci are known to produce a variety of antimicrobial substances. Soil samples were collected from the Bougtob region in El-Bayadh city (34°02'32.7"N 0°05'21.4"E), using the classic method of Pochon and Tardieux 1962. Thirty isolates with the same Nocardiopsis morphology were isolated on 65. GYM Streptomyces medium using dilution techniques, and tested for their ability to produce antimicrobial agents. The morphological and biochemical tests were used to identify thirty isolates of the Nocardiopsis genus. The antimicrobial activity of the compound produced by the Rhodococci strain was tested by the cross-streak assay method, against the Gram-positive bacteria (Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus) and the Gram-negative bacteria (Escherichia, coli). Out of thirty strains, two showed broad-spectrum activity. The strains were identified using various biochemical tests. These results indicate that the Algerian Sahara soil microorganisms could be an interesting source of antibacterial and antifungal bioactive substances.

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