Simplification of the Culture Medium and Action of The Bioreactor Material on The Stimulation of Chlorella vulgaris Production

Samia, Ghomari,; Yasmina, Benabdesslem,; Chafik, Mhamdia,; Samia, Daoud Hamdi,; Meghit Boumediene, Khaled,; Kouider, Cherifi,

doi:10.21608/eajbsc.2022.260478

Simplification of the Culture Medium and Action of The Bioreactor Material on The Stimulation of Chlorella vulgaris Production

Document Type : Original Article

Authors

¹ Laboratoire de Nutrition, Pathologie, Agro-Biotechnologie et santé. Djillali Liabès university, Natural and Life Sciences faculty, BP 89, 22000 Sidi-Bel-Abbès, Sidi-Bel-Abbès, Algéria.

² Laboratoire de Nutrition, Pathologie, Agro-Biotechnologie et santé. Taher Moulay University of Saida. Faculty of Natural and Life Sciences, Algeria.

³ Laboratoire de Nutrition, Pathologie, Agrobiotechnologie et Santé, Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi-bel-Abbes, Algeria.

⁴ INRAA-Ouest, Sidi Bel Abbes. Algeria

⁵ Laboratoire de Nutrition, Pathologie, Agrobiotechnologie et Santé, Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi-bel-Abbes, Algeria

⁶ Laboratoire de Valorisation de la Biodiversité; et Conservation des Plantes, Djillali Liabès University, Faculty of Natural and Life Sciences Sidi-Bel-Abbes, Algeria.

10.21608/eajbsc.2022.260478

Abstract

Cultivation Chlorella vulgaris still remains a challenge for scientists against the background of the cultural systems and the different cultural media deployed. In this context, our study aimed to a comparison of culture media BG11 known for its validity in the culture and new media GS1 and GS2 (rich in ammoniacal and nitric nitrogen) easy to prepare and used. Further culture parameters were involved such as photoperiod (16 h and 24 h) and the materials of the culture contents used in the bioreactor (glass bottles, plastic bottles in LDPE (low-density polyethylene) and plastic bottles in PET (polyethylene terephthalate)). The obtained results indicated that these parameters displayed a direct effect on the productivity of the culture and that their effect was mutual. However, for optimal production of C.vulgaris biomass, it is recommended to use a GS1 culture medium at a photoperiod of 24h, for a production of 50.6 x 10⁶ cells/ml, and fresh biomass of 0.35 g/L. It is however advised to respect the different types’ rates of nitrogen in order to avoid cell division inhibition. Therefore, a concentration of 0.13 g/L of nitric nitrogen and 0.28 g/ L of ammoniacal nitrogen is required, with a low dose of urea nitrogen of 0.34g/ L.
The use of PET plastic in the bioreactor restricts the adhesion of the culture to the cylinders’ walls, and with reduced loss rate, a homogeneous diffusion of the light in the totality of the cultures, and a lifetime of the bioreactor remains greater.

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