Valorization of Essential Oil of Rosmarinus Officinalis L. from El Bayadh Region-Algeria

Document Type : Original Article

Authors

1 Valorisation des Phyto-Ressources et Ecodéveloppement des Espaces, Djillali Liabes University of Sidi Bel Abbés 2200, Algeria - Department of Environnement, Djillali Liabes University of Sidi Bel Abbés 2200, Algeria.

2 Valorisation des Phyto-Ressources et Ecodéveloppement des Espaces, Djillali Liabes University of Sidi Bel Abbés 2200, Algeria - Institut of Science Nour Bachir University Center. El bayadh Algéria 32000

3 Laboratory of Plant Biodiversity; Conservation and Valorization, Djillali Liabes University of Sidi Bel Abbés 2200, Algeria - Department of Environnement, Djillali Liabes University of Sidi Bel Abbés 2200, Algeria.

Abstract

Our work focuses on endorsing the biological effects of Rosmarinus Officinalis L essential oils from the arid zone in Algeria by studying its chemical composition, and biological activities; in vitro and in vivo. The chemical compounds of the essential oil have been identified by gas chromatography and mass spectrometry. Thirty-two constituents, accounting for 99.97% of the total oil composition were identified. the main compounds of the essential oil were Eucalyptol (70,90%), Borneol (16.63%), ⍺-Pinene (10,52%) β- Pinene (5,77%), Camphor (2,15 %), and ⍺-terpineol (1,45%), respectively. The antioxidant activity of the hydro distilled oil was studied using DPPH to determine IC50 of 18.04μg/ml. The antibacterial activity of essential oils was evaluated against five microorganisms using the diffusion disc method on bacteria: Candida Albicans ATCC10231, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853), Staphylococcus aureus ATCC 25923, Bacillus ATCC 11778,diameters vary between 6 and 19 mm. The toxicity was tested on Winstar rats; for a dose less than or equal to 2000mg/kg no toxicity signs were shown. In vivo, the Anti-Inflammatory properties were evaluated using the Induction of edema by carrageenan, the essential oil has a great effect at the concentration of 600mg /kg.

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