Micronucleus and Comet Assay as An Index for Carbon Tetrachloride Genotoxicity in Rats

Document Type : Original Article

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Zoology Department, Women’s College for Arts, Science and Education - Ain Shams University- Egypt

Abstract

Many studies have reported that green coffee extract (GCE) has many benefits such as anti-diabetic, antioxidant, antihypertensive, and anti-obesity effects. The hepatoprotective effect of Green Coffee Extract (GCE) and olive oil in liver rats were studied as a result of the treatment with Carbon Tetrachloride (CCL4) compared to that of control. There was a sharp increase in tail DNA% from the GCE group to the CCL4 group, which reached the beak in the CCL4 group (24.67+5.09) (P<0.001), after being treated with GCE with CCL4, the chart declined to (14.67+2.68). CCL4 group induced a very high frequency of MN (14.00+1.67) compared to the control one (1.83+0.75). While animals treated with GCE at the same time with the CCL4 group induced a significant reduction in the number of MN, 38/6000 (6.33+1.21). The results of this trial illustrated the protentional antioxidant effects of Green Coffee Extract and Olive Oil together or apart on the hepatotoxicity and cancerous effects of Carbon Tetrachloride in the liver and bone marrow of male albino rats. So, it was concluded that the GCE and olive oil minimizes the genotoxic role of CCL4 in rats.

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