Persistence of Aedes Aegypti and Molecular Detection of DENV In Mosquitoes in Red Sea Governorate, Egypt

El-Ansary, Ramy E.; Bream, Ahmed S.; El-Dabae, Wahid H.; Mahmoud, Shaymaa H.

doi:10.21608/eajbsc.2022.231071

Persistence of Aedes Aegypti and Molecular Detection of DENV In Mosquitoes in Red Sea Governorate, Egypt

Document Type : Original Article

Authors

¹ Zoology and Entomology Department, Faculty of Science, Al-Azhar University, Egypt

² Microbiology and Immunology Department, Veterinary Research Division, National Research Centre, Dokki 12622, Giza, Egypt.

³ Zoology Department, Faculty of Science, Menoufia University, Egypt.

10.21608/eajbsc.2022.231071

Abstract

Aedes aegypti (L), (Diptera: Culicidae) is a major vector for the spread of several dangerous arboviral diseases, including Dengue Fever. Dengue fever (DF) is one of the most common mosquito-borne viral zoonosis, affecting over 100 countries worldwide. Dengue fever (DF) and dengue haemorrhagic fever (DHF) are caused by four dengue viruses serotypes (DENV-1 to DENV-4). The purpose of this study was to determine the prevalence of Ae. aegypti mosquitos and their dengue virus carriers in Egypt's Red Sea governorate between 2019 and 2020. From September to December of 2019 and 2020, 3200 fourth larval instar mosquitoes and 1600 adult mosquitoes were collected and divided into 16 pools from 8 different regions associated with the Red Sea governorate. In addition to the standard morphological key, a molecular study was carried out using Cytochrome oxidase (COI) gene-specific primers. By using a PCR technique, all Ae. aegypti larvae and adults were tested for the presence of DENV. All pools collected from larvae and adults tested negative for DENV, indicating that, Ae. aegypti does not harbour DENV.

Keywords