Screening the Antioxidant Activity of Aqueous and Ethanol Extracts from Fenugreek (Trigonella foenumgraecum) Seeds in Vitro

Hussein, Anmar Sael; Salman, Safa Salah; Al-mashtaa, Hind Isam Abdullah; Abdullah, Muna Ahmed; Al-Mudallal, Nada H. A.L.; Al-Abbasi, Shaima Hassan Ali; Al-Nasseri, Saja Jamal Noman

doi:10.21608/eajbsc.2018.22665

Screening the Antioxidant Activity of Aqueous and Ethanol Extracts from Fenugreek (Trigonella foenumgraecum) Seeds in Vitro

Document Type : Original Article

Authors

¹ College of medicine, Faluja University.

² Biology department , College of education, Iraqia University

³ College of density, Tikrit University, Branch basic Science

⁴ Microbiology department, College of Medicine, Iraqia University

⁵ University of samara

⁶ University of Tikrit

10.21608/eajbsc.2018.22665

Abstract

In this study was to prepare extracts aqueous and ethanol from Fenugreek Trigonella foenumgraecum Seeds powder, The aqueous extract and ethanol extract were used for analyzing the main phytochemical, nutrient and active groups composition of fenugreek seeds powder Trigonella foenumgraecum.The preliminary tests of active groups in extracts were carried out. It appeared to contain (alkaloids, flavonoids ,phenolic compounds , tannins ,terpenes and saponins) . The extracts were different in their content of active groups quantitatively and qualitatively. And to evaluate in vitro antioxidant activity of aqueous and ethanol seeds extracts of Trigonella foenumgraecum.,was used three different methods and all the three methods have proven the effectiveness ofextracts aqueous and ethanol from Fenugreek Trigonella foenumgraecum Seeds compared to the reference standard antioxidant ascorbic acid, Showed the study is conducted to estimate the activity of the extracts prepared from Fenugreek Trigonella foenumgraecum Seeds as antioxidant in vitro by measuring the reducing power and their capacity of scavenging hydrogen peroxide as compared with the standard compound ascorbic acid. The results indicated that the extracts aqueous and ethanol Fenugreek Trigonella foenumgraecum Seeds exhibited the highest had a reducing power and a capacity of scavenging hydrogen peroxide, and demonstrated that extracts antioxidant capacity less than the standard for the reducing power and phosphomolybdate reduction methods. This is observed through increasing the intensity of absorbance with increasing the concentration.

Keywords