Document Type : Original Article
Authors
1
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
2
Department of Pathology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
Abstract
Adriamycin (ADR) is an effective antineoplastic agent commonly used to treat different types of cancer. The use of ADR in clinical chemotherapy is limited due to diverse toxicities, including gonadotoxicity. The present study was designed to evaluate the effect of nano-magnetic particles (NFe3O4) and Graviola leaves extract and (NFe3O4 + Graviola leaves extract) against ADR -induced gonadotoxicity. The rats divided into 5 groups (10 rats per group). The first group (normal g.), the second group received ADR alone (5 mg/kg body weight, i.p.) as (control g.), the third group received ADR followed by NFe3O4 (5 mg/kg body weight, i.p.), the fourth group received ADR followed by Graviola (200mg/kg body weight, orally), and the fifth group received ADR followed by NFe3O4(5 mg/kg body weight, i.p.) and Graviola leaves extract(200mg/kg body weight, orally). Animals were sacrificed 36 days after treatment and sex hormones (Testosterone and F.S.H) in serum. Also, the activities of antioxidants such as (SOD), (CAT), (GSH) and the level of (MDA) as a marker of lipid peroxidation were measured. In the ADR-exposed rats, Testosterone, F.S.H, GSH, SOD and CAT were significantly decreased, while MDA was significantly increased.When compared with normal animals. In the Group NFe3O4, graviola, and (NFe3O4 +graviola), Testosterone, F.S.H, GSH, SOD and CAT enzymes levels were significantly increased, while MDA level was significantly decreased when compared with ADR treated animals. The results showed that there is a possibility that the ethanolic extract of graviola leaves and NFe3O4 ameliorate the gonadotoxicity induced by ADR in rats.
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