Characterization of White Button Mushroom and Its Biomedical Applications

M., Mohammed Salaheldin; Easa, Saadia M.; Badr, Mohamed S.

doi:10.21608/eajbsc.2022.215688

Characterization of White Button Mushroom and Its Biomedical Applications

Document Type : Original Article

Authors

¹ Microbiology Department, Faculty of Science, Ain Shams university

² Department of Molecular Biology, Medical Research Centre, Faculty of Medicine, Ain Shams University

10.21608/eajbsc.2022.215688

Abstract

The biological contents of the white button mushroom including(lectin and polysaccharides) were extracted from its dried fruiting bodies and purified through Q-Sepharose column which was considered a strong anion for adsorption of lectin with high performance. The purified lectin has a great hemagglutinating activity (HA) that was considered a specific feature for lectin and inhibited by GlcNAc which was a specific binding sugar receptor. In this study, we reported that lectin agglutinated all blood groups without specificity, was active below 70 and 80°C and it had no activity at that temperature degrees. The best pH media for HA within range (6 to 9) for 24hr the purified lectin had molecular weight 16KDa that was determined by SDS PAGE ELECTROPHORESIS. Gene expressions by RT-PCR of the white button mushroom showed that thelectin upregulated Bax (pro-apoptotic gene). Lectin had apoptotic activity on HepG-2 cell line. The cell toxicity assay showed that mushroom’s lectin has high toxicity on malignant cells with very low toxicity on WI-38 (normal cells). White button mushroom lectin had antiproliferative & toxic effects on HepG-2 with IC₅₀; 41.90 ug/ml while had very weak toxicity on WI-38(normal cells) with IC₅₀(98.54 ug/ml). The lectin of edible mushroom can be utilized in human eating regimens and recommended for malignancy with a safety margin in clinical pathological research with different concentrations starting from 200g fruiting bodies mushroom & 98.44 ug/ml lectin concentration.

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