Effect of a single dose of a bradykinin potentiating factor isolated from scorpion venom (Buthus occitanus) on total protein and albumin in serum of irradiated growing male Guinea pigs.

Whole body gamma irradiation of growing male Guinea pigs at the dose of 150 rads/animal reduced the levels of total protein and albumin.  Growing Guinea pigs males (250 ± 30 gm. b. w.) were divided into two main groups; non-irradiated and irradiated groups and every main group were divided into four sub-groups. Irradiated growing Guinea pigs males groups were exposed to 150 Rads/animal by cesium-137 irradiation unit with a dose route of 1.06483 rad per second in a uniform manner and divided into four sub-groups. In irradiated groups; the first sub-groups were interaperitoneally (i.p) injected with100 µL saline solution. The second sub-groups were i.p. injection of bone marrow cells from their twins (2.5x10⁶ cells/animall). The third sub-groups injected i. p with BPF (1µgm/gm. b. w.) which was dissolved in saline solution.
The fourth sub-group injected with i.p solution of BPF (1µg/gm. b. w.) together with bone marrow cells from their twins (2.5x10⁶ cells/animal). The non-irradiated sub-groups were injected with the same manner of irradiated sub-groups. The level of total protein and albumin in serum were measured at periods of 1, 3, 7, 14 and 21 days. Irradiated animals i.p. injection of BPF (single dose); appears slight to improve the changes which occurred in both total protein and albumin compared to irradiated sub-group. However, irradiated Guinea pigs injected with single dose of BPF together with bone marrow cells were more improvement of these parameters than sub-groups injected with only single dose of BPF. The improvement of these parameters may be attributed to stimulate growth factors and/or growth hormones to increase protein synthesis as an effect on the liver that necessitates future investigations.