Nuclear receptor FTZ: partial cloning and expression pattern in fat body of the American cockroach Periplaneta americana (Linnaeus, 1758) during vitellogensis

Elgendy, Azza Mohamed; Takeda, Makio

doi:10.21608/eajbsc.2012.16134

Nuclear receptor FTZ: partial cloning and expression pattern in fat body of the American cockroach Periplaneta americana (Linnaeus, 1758) during vitellogensis

Document Type : Original Article

Authors

Azza Mohamed Elgendy ¹
Makio Takeda ²

¹ Entomology Department, Faculty of Sciences, Cairo University, Egypt, Giza, P.O. Box 12613

² Insect Science Laboratory, School of Agricultural Science, Kobe University, Kobe, Japan

10.21608/eajbsc.2012.16134

Abstract

The
aim of the present study was to understand the importance of the nuclear
receptor Fushi tarazu factor 1 (FTZ-F1) in relation to vitellogenin genes
expression in the fat body of Periplaneta
mericana during the first vitellogenic cycle. Initially, a 342 bp fragment
was cloned from the female fat body through degenerate primer RT-PCR method.
Its sequence analysis revealed high homology with other insects and animals
FTZ-F1. In order to find the relation between the P. americana FTZ and vitellogenins, we analyzed the expression
pattern of the latter in the fat body during vitellogensis. The PamFTZ transcript was detected during all
stages of vitellogenesis in the fat body with a little increase in day 5, preceding
the bulk of vitellogenin genes expression. We assumed that nuclear protein FTZ
in the hemimetabolous insect, P.
americana might functions as a competence factor that facilitates juvenile
hormonal activation of gene expression as in holometabolous insect, Drosophila melanogaster.

Keywords