Biochemical studies of Bacillus Thuringiensis var.kurstaki, Serratia marcescns and Teflubenzurone on cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptea: Noctuidae)

Document Type : Original Article

Authors

1 Plant Prot. Res. Inst., Agric Res. Centre, Dokki, Giza, Egypt

2 Faculty of Agriculture, Ain-Shams- University, Egypt

Abstract

Two biopesticides Serratia marcescens [Eubacteriales: Enterobacteria] (used at MC50, concentration caused 50% malformation) and Bacillus thuringiensis Var. kurstaki (used at LC50) and insect growth regulator Teflubenzuron (used at LC 50 value) were used for treatment of 2nd instar larvae of cotton leafworm, Spodoptera littoralis (Boisd.). Sequential combined Effect was carried out bytreating 2nd instar larvae with LC50   value of B. thuringiensis or Teflubenzuron then the larvae allowed to pupate on sawdust treated with S. marcescens at MC50  .The effect of these three agents were assessed by toxicity The obtained LC50 valueswere 0.113 and 165.64 ppm, for Teflubenzuron and B. thuringiensis Var. kurstaki. (protecto), respectively. Moreover, the activity of Chitinase, protease, trehalase, and the main components (total lipids, proteins and carbohydrates) were significantly fluctuated during the different periods of pupal stage.

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