Changes of cotton leaf worm haemocytes and esterases after exposure to compounds derived from urea and Rice Straw

Hassan, Heba A.; Bakr, Reda F.A.; Abd El-Bar, Marah M.; Nawwar, Galal A.; Elbanna, Heba M.

doi:10.21608/eajbsc.2013.16094

Changes of cotton leaf worm haemocytes and esterases after exposure to compounds derived from urea and Rice Straw

Document Type : Original Article

Authors

¹ Plant Protection Institute, Agricultural Research Center

² Entomology Departments, Faculty of Science, Ain Shams University

³ Applied Organic Chemistry Department, National Research Centre

10.21608/eajbsc.2013.16094

Abstract

In the current study, the role of cotton leaf worm haemocytes and esterases in detoxification were recorded. The haemolymph was collected from the 6^th instar larvae which treated with LC₂₅, LC₅₀, LC₇₀of three newly compounds extracted from wastes from natural origin, Cyano acetyl urea (CAU), Benzimidazolyl acetyl urea (BAU) from urea and Cyano acetyl urea (CAH) from rice straw as 4^th instar larvae. The mean of total haemocytes counts in haemolymph of untreated 6^th larval instar was 9430±35.78 cells/mm³. Data indicated an increase in the total haemocytes of treated ones with CAU, at LC₂₅ and LC₅₀, BAU at LC₂₅and CAH at LC_25,LC₅₀of CAH the percentages of change were: 19.01, 14.31, 1.90, 21.00 and 14.55, respectively. In addition, the percentages of oenocytoid counts were increased in all concentrations for all compounds except for CAH (at LC₇₀) as it did not change comparing control count. On contrast, data cleared reductions in granulocytes in all concentrations for all compounds except for BAU at LC50 as they increased slightly (15.12%). On the other hand, the results were showed great differences in number of zones of esterase activity and in substrate specificity between treated and untreated samples. For instance, in the case of α - naphthyl acetate, 36 esterase bands were detected in control and treated larval samples with Rf ranging between 0.01 to 0.28. While, in the case of b - naphthyl acetate, 39 esterase bands were detected in control and treated larval samples with Rf ranging between 0.01 to 0.92.

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