Comparative Physiological Studies on the Effect of Nano-magnetic Particles (iron oxid) and Graviola Leaves Extract on Adriamycin Induced Cardiotoxicity in Male Albino Rats

Elshater, Abd-Elraheim A.; Ali, Rana A.; Gedawy, Huda Y.

doi:10.21608/eajbsc.2016.13680

Comparative Physiological Studies on the Effect of Nano-magnetic Particles (iron oxid) and Graviola Leaves Extract on Adriamycin Induced Cardiotoxicity in Male Albino Rats

Document Type : Original Article

Authors

Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt

10.21608/eajbsc.2016.13680

Abstract

Adriamycin (ADR) or Doxorubicin (DOX), is an effective antineoplastic agent commonly used for the treatment of various cancers. ADR is an anthracycline chemotherapeutic containing a quinone group that is known to produce reactive oxygen species (ROS) in heart. . Because of its cardiotoxicity side effects in various tissues, which lead to cardiomyopathy and congestive heart failure, the clinical application is limited. The present study was designed to evaluate the cardioprotective effect of nano-magnetic particles (NFe3O4) and Graviola (Annona muricata) leaves extract and combination of them against ADR -induced cardiomyopathy in rats which are divided into 5 groups including one control and four experimental (10 rats per group). They received saline (normal), ADR alone (15 mg/kg body weight, i.p.) (control), ADR followed by NFe3O4 (15 mg/kg body weight, i.p.), ADR followed by Graviola (200mg/kg body weight, oral), and ADR followed by NFe3O4 and Graviola. Animals were sacrificed 28 days after treatment and evaluations were made by measuring cardic enzymes (CK and LDH) in serum. Also the activities of antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) as well as the level of malnodialdhyde (MDA) as a marked of lipid peroxidation . In the ADR-exposed rats,CK, LDH, and MDA significantly increased, while GSH, SOD and CAT enzymes decreased when compared to normal animals. In the Group NFe3O4, graviola leaves extract, and (NFe3O4 +graviola leaves extract alone), CK, LDH and MDA levels significantly decreased, while GSH, SOD and CAT enzymes levels significantly increased when compared to ADR treated animals. The results showed that there is a possibility that the ethanolic extract of graviola and NFe3O4 and combination of them ameliorate the toxicity induced by ADR in rats.

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