Associations Between Levels of Liver Enzymes and Circulating Antigens in Individuals Infected with Schistosomiasis Heamatobium

Zahran, Ibrahim A. E.; Zaahkouk, Samir A. M.; Aly, Ibrahim R.B.; Samn, Alaa A. M.; EL-Shamy, Sawsan A.M.

doi:10.21608/eajbsc.2020.134903

Associations Between Levels of Liver Enzymes and Circulating Antigens in Individuals Infected with Schistosomiasis Heamatobium

Document Type : Original Article

Authors

¹ Zoology Department, Faculty of Science, AL-Azhar University

² Department of Parasitology, Theodor Bilharz Research Institute

³ General Biology, Basic Science Center, Misr University for Science and Technology

10.21608/eajbsc.2020.134903

Abstract

Current estimates of the prevalence of schistosomiasis depend on the use of well-established, but imperfect, diagnostic tests where specimens are processed by routine parasitological methods and parasitological cure is judged by inspection of a cohort of children before and after Praziqantel (PQZ) treatment. In this study, in order to evaluate the different immunodiagnostic antigen detection assays, the selection of a proper antigen and its purification followed by propagation of its specific antibodies and purification were mandatory. Measurement of Liver function and kidney function were performed and its relation to S. heamatobium infection in different age and sex individuals were studied. Serum samples from an infected human with S. haematobium gave a strong reaction against S. haematobium SWAP antigen and no cross-reacting. with other parasites. The produced anti-S. haematobium SWAP IgG-pAb diluted 1/250 in PBS/T buffer gave strong reactivity to S. haematobium SWAP. The highest percentages of S. haematobium infected patients were in the age group 21-30 years (25%) and the least percentages of patients were in the age group of 51-55 years (2.9%). Levels of ALT , AST and ALP in the serum of humans among schistosomiasis group recorded a highly significant increase compared to the healthy control group. In conclusion strong association between biochemical parameters and serum circulating antigens among schistosomiasis infection.

Keywords