Effect of Heavy Metals Levels In Follicular Fluid on ICSI Outcome

El Mohr, Mohamed F.; Faris, Mohamed; Bakry, Sayed; Hozyen, Heba; Elshaer, Fathy M. M.

doi:10.21608/eajbsc.2020.125100

Effect of Heavy Metals Levels In Follicular Fluid on ICSI Outcome

Document Type : Original Article

Authors

¹ Dr Faris Medical Center for Infertility and Human Reproduction, Heliopolis, Cairo, Egypt

² Laboratory of Molecular Biology and Embryology, Zoology Department, Faculty of Science, Al-Azhar University Nasr City, Cairo, Egypt

³ Animal reproduction & Artificial Insemination Department, Veterinary Research Division, National Research Centre, Dokki, Cairo, Egypt

⁴ Department of Biology, College of Science, Jouf University, Sakaka. Saudia Arabia, -Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

10.21608/eajbsc.2020.125100

Abstract

Heavy metals and trace elements adversely affect animal health and the reproductive system and its functions through direct or indirect effects on numerous organs and systems. This study was aimed to evaluate the effect of heavy metals levels in follicular fluid on a patient undergoing Intracytoplasmic Sperm Injection (ICSI) outcomes namely Cadmium (Cd), Lead (Pb), and Arsenic (As). In comparison between the normal concentration level of Cd and the high concentration level of Cd the present study showed a significant difference in embryos quality (P=0.01611), while no statistically significant difference in pregnancy rate was observed. Although Pb showed a significant difference in embryos quality; grade I embryos in normal level concentration than high-level group (P=0.00021), and grade III embryos were significant in a high-level concentration of Pb than low-level group (P=0.0043). The present study concluded that a high level of heavy metal concentration in the follicular fluid has a harmful effect on the grading of embryos and these lead to a decrease in the chance to select the beast embryos before transferred (Cd and Pb).

Keywords