2024-03-29T16:25:03Z
https://eajbsc.journals.ekb.eg/?_action=export&rf=summon&issue=3454
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Purification of perforin and study expression of perforin in leukemia patient
Najwa
Ahmed
Amal
Fayyad
Salimeh
Mohammadi
Subhi
Hamza
Abdul
Al-Faisal
This work aimed to study compare of extraction, purification and study gene expression of perforin from ALL, CLL and healthy, to chive this goal, blood samples were collected from 10 Leukemia cases (5 ALL and 5 CLL) and 5 healthy. Comparison of the molecular weight of purified perforin from these groups shows no detectable differences as compared with that of standard perforin which specified as 70KDa using SDS-PAGE. However, gene expression by using western blotting, it was found that the level of perforin in these groups were different due to differential gene expression between patients, and healthy control. Hence high levels were found in healthy individuals and CLL patient as compared with ALL patients as expressed by the presence of intense band.
perforin
leukemia patient
2013
12
01
1
9
https://eajbsc.journals.ekb.eg/article_16090_bf303c9700949d9ac296a8a88940b0bb.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Biological and molecular effects of pyriproxyfen, insect growth regulator (IGR), on Met gene in susceptible wild type and Met mutant Drosophila
Reda
Bakr
Jehane
Hafez
Thorya
Alnagar
Dalia
Salem
Ola
Zyaan
Juvenile hormone (JH) signaling pathway at the molecular level is still not well understood. Methoprene-tolerant (Met) in Drosophila melanogaster fulfills many of the requirements for a hormone receptor gene. However, Met mutant flies are resistant to insect growth regulators (IGRs). In our study, the resistance to juvenile hormone agonist (JHA) in Methoprene-tolerant (Met) mutant flies was studied. Met mutant and susceptible wild type larvae were treated by different concentrations of pyriproxyfen at 0, 0.03, 0.1, 0.3, 1, and 3 ppm. We observed that the mean percentage mortalities in Met mutant were significantly (P<0.05) lower than that in the susceptible wild type. Also, resistance in insects to IGRs due to Met gene was studied using PCR and DNA sequencing analyses. We found that there are no changes in the DNA sequences of Met gene after the treatment with pyriproxyfen.
pyriproxyfen- Met gene- Drosophila melanogaster
2013
12
01
11
18
https://eajbsc.journals.ekb.eg/article_16091_c006d3ea2c0195656045bdeef21a86cc.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
The Activity of Some Detoxification Enzymes in Spodoptera Littoralis (Boisd.) Larvae (Lepidoptera – Noctuidae) Treated With Two Different Insect Growth Regulators
Reda
Bakr
Mona
Abd Elaziz
Nehad
El-barky
Mohamed
Awad
Hisham
Abd El-Halim
The susceptibility to IGRs, moulting hormone agonist, mimic (tebufenozide) and chitin synthesis inhibitors match (lufenuron) was studied in the 2nd and 4th larval instars of the cotton leafworm Spodoptera littoralis (Bosid). Obtained results revealed that match was the more toxic against 2nd larval instars at sub-lethal concentrations LC25 and LC50 (0.3 and 0.6 ppm, respectively) than mimic (1.1 and 1.5ppm, respectively). But LC90 level was the same in the two IGRs. With respect to 4th larval instar, match induced the higher toxic effect at all sub lethal doses than mimic. Biochemical analysis showed that match and mimic exhibited a severe reduction in the activities of the detoxification enzymes, acid phosphatase and esterases (α and β), as compared to the control. The enzymatic activities were inhibited with the increase in the time post-treatment and also with the increase in dose. Therefore, the tested IGRs, tebufenozide and lufenuron , may be not detoxify by these enzymes.
IGRs
toxicity
detoxification enzymes
Spodoptera littoralis
2013
12
01
19
27
https://eajbsc.journals.ekb.eg/article_16092_b177851482e41f636f0cb8512b2c2833.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Effect of renal dialysis on some haematological, electrolytes and biochemical parameters in hepatitis patients.
El–Zawhry
I.
Salem
M.
Abdel-Rached
H.
Wafeek
M.
Galal
S.
Mohamed
T.
The present study was designed to finding the influence of renal dialysis in some haematological, electrolytes and biochemical parameters in kidney failure and kidney failure with hepatitis patients (C and B) virus. The experiment was designed from ten normal healthy patients as control group and sixty male human patients were selected from hospital campus of the ZagazigUniversity conducting adialysis three time aweek and classified according to dialysis into two major groups, the first group: patients with less than one year of dialysis. The second group: patients with more than one year of dialysis. Each group are divided into three sub groups are renal failure, renal failure with hepatitis c virus (HCV) and renal failure with hepatitis B virus (HB), asignificant decrease was recorded in RBCs count, Hb, HCt and platelets count, also asignificant decrease was showed in serum sodium and calcium levels, while asignificant increase was demonstrated in WBCs count, serum potassium, phosphorus levels and transaminases (ASAT and ALAT) enzymes activities in patients with renal failure and renal failure with hepatitis (C and B) virus .
renal failure
Hemodialysis
Hepatitis
electrolytes
Kidney function and Liver function
2013
12
01
29
34
https://eajbsc.journals.ekb.eg/article_16093_3da3f67a423ee3962e4c110f694925eb.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Changes of cotton leaf worm haemocytes and esterases after exposure to compounds derived from urea and Rice Straw
Heba
Hassan
Reda
Bakr
Marah
Abd El-Bar
Galal
Nawwar
Heba
Elbanna
In the current study, the role of cotton leaf worm haemocytes and esterases in detoxification were recorded. The haemolymph was collected from the 6th instar larvae which treated with LC25, LC50, LC70 of three newly compounds extracted from wastes from natural origin, Cyano acetyl urea (CAU), Benzimidazolyl acetyl urea (BAU) from urea and Cyano acetyl urea (CAH) from rice straw as 4th instar larvae. The mean of total haemocytes counts in haemolymph of untreated 6th larval instar was 9430±35.78 cells/mm3. Data indicated an increase in the total haemocytes of treated ones with CAU, at LC25 and LC50, BAU at LC25 and CAH at LC25, LC50 of CAH the percentages of change were: 19.01, 14.31, 1.90, 21.00 and 14.55, respectively. In addition, the percentages of oenocytoid counts were increased in all concentrations for all compounds except for CAH (at LC70) as it did not change comparing control count. On contrast, data cleared reductions in granulocytes in all concentrations for all compounds except for BAU at LC50 as they increased slightly (15.12%). On the other hand, the results were showed great differences in number of zones of esterase activity and in substrate specificity between treated and untreated samples. For instance, in the case of α - naphthyl acetate, 36 esterase bands were detected in control and treated larval samples with Rf ranging between 0.01 to 0.28. While, in the case of b - naphthyl acetate, 39 esterase bands were detected in control and treated larval samples with Rf ranging between 0.01 to 0.92.
Spodoptera littoralis- urea derivatives
rice straw- detoxification- haemocytes
esterase patterns
2013
12
01
35
48
https://eajbsc.journals.ekb.eg/article_16094_ab51285f3370a895376a39fa51ce4a43.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Immunochemical studies on phospholipase A2 from Naja nigricollis venom
Ahmed
Wahby
Amr
El Hakim
El Sayed
Mahdy
Walaa
Salama
Four PLA2 isoenzymes (N. nigricollis CM-PLA2I-IV) were purified from Naja nigricollis (N. nigricollis) venom using Sephadex G-50 gel filtration, and ion exchange chromatography on CM-Sephadex C-25. The characterization of the isolated PLA2 isoenzymes revealed similarities in molecular weights, and differences in the isoelectric points, the optimum temperature, optimum pH, optimum Ca+2concentration and metal ion requirements. A good correlation (r <0.7) for the in vitro neutralization of enzymatic PLA2s activity and the ELISA titers was found for sera collected at one week from each boosting of the rabbits. The found correlations were particularly high (r>0.9) when the purified Seph-PLA2 and CM-PLA2II were used rather than the whole venom. The established correlations show the importance of the purified PLA2 enzymes as immunogens for raising therapeutic antisera and as diagnostic reagents for in vitro determination of the potency of the therapeutic antisera.
Antivenom
correlation
Naja nigricollis
Phospholipase A2
Snake venom
2013
12
01
49
66
https://eajbsc.journals.ekb.eg/article_16095_eef0fe7095afb7601f4ba50f99176289.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Protective effect of oregano water extract against Carbon tetrachloride induced Hepatotoxicity in female rats
Muhammad
El-Shafeey
Tarek
Taha
Carbon tetrachloride (CCL4) is one of the most toxic chemicals causes oxidative stress in the living organisms. Our experiment started with twenty female rats that were divided into 4 groups; The 1st groupG1 served as control group, 2nd group G2 injected twice a week with CCL4 diluted in olive oil (1:1 v/v), 3rd group G3 were fed daily for 14 days with 1ml of Oregano water extract and the 4th group G4 were injected with CCL4 plus daily feeding with 1ml of Oregano water extract. The biochemical results indicated a significant elevation in the liver and kidney functions including ALT, AST, creatinine, uric acid and urea for the 2nd group due to the toxicity induced by the CCL4. The levels of ALT, AST, creatinine, urea and uric acid were significantly decreased after the administration of oregano water extract in the 4th group. Lipid profile was evaluated in the four groups by measuring the levels of total cholesterol, HDL-C and triglycerides. The variation noticed by the over and down expression of the liver genes due to CCl4 was explored. These up and down regulated genes were tracked using differential display technique.
Oregano water extract
Carbon tetrachloride
Hepatotoxicity
Rats
2013
12
01
67
76
https://eajbsc.journals.ekb.eg/article_16096_bc414696ddeac9bcc59a5700fce3db31.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Purification, Characterization and Molecular Studies on Oxalate Decarboxylase from B. cepacia inhabits Egyptian soil
Hala
Abou Shady
Mona
Kilany
Qadria
Genedi
Wafaa
Farouk
Oxalate decarboxylase (OXDC) is an intracellular enzyme that decomposes toxic oxalate into formate and CO2. So, it is used for medical diagnosis of oxalate and decreasing the oxalate level. OXDC enzyme was purified from B. cepacia, characterized and subjected to amino acid analysis. Genomic and plasmid DNA from B. cepacia were purified. PCR products of OXDC gene was subjected to DNA sequence analysis. OXDC was purified 18.05 folds to near homogeneity with a specific activity of 4.496 U/ μg protein and showed pure single band with molecular weight 30 kDa. OXDC showed an optimal activity at pH 6, 35C° using Zn+2 and Mn+2 as a catalyst. The most abundant amino acid is alanine and the less frequent amino acid is cysteine. Comparing the genomic and plasmid DNA showed that both have the same size. Similarity search results showed that the obtained sequence can detect OXDC gene in many B. cepacia genomes.
This work is supported byNational Organization for Drug Control and Research (NODCAR), Cairo, Egypt
Oxalate decarboxylase
plasmid
genomic DNA
B. cepacia
2013
12
01
77
86
https://eajbsc.journals.ekb.eg/article_16097_bde53b859156ba2e31514a33f93b6985.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Modulation of autophagy enhances the apoptotic cell death in human T lymphoma cells treated with Anthocyanin
Gamal
El-Sokkary
Douaa
Sayed
Salwa
Fuad
Abo Bakr
Abdel Shakor
In the present study, the effect of modulation of autophagy induced byanthocyanin on cell death of Human T lymphoma cells (Jurkat) was studied.Anthocyanin was abstracted from dry petals of Hibiscus sabdariffa,aquous solution of anthocyanin was added to cells at different concentrationsthen cell viability was determined by trypan blue exclusion method. Autophagyinduced by anthocyanin was inhibited by 5 mM NH4CL which haltslysosomal enzymes and accordingly preventing autolysosomes formation. On theother hand, Autophagy was enhanced by glucose starvation. In both experimentsof autophagy inhibition and enhancement, cell viability was studied toinvestigate the effect of autophagy modulation on cell viability. The resultsof this work revealed that both inhibition and enhancement of autophagy inducedby anthocyanin lead to massive cell death. Immuno-detection of active caspase3, one of the major hallmark of apoptotic cell death revealed remarkableincrease of active caspse3 upon autophagy inhibition or enhancement. Inconclusion, modulation of autophagy induced by anthocyanin lead to increasingof apoptotic cell death
modulation of autophagy- human tlymphoma cells
Anthocyanin
2013
12
01
87
97
https://eajbsc.journals.ekb.eg/article_16098_81ff41c5105ffe84f758147426910055.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes
Reda
Bakr
Mohamed
Gesraha
Noha
Guneidy
Nagy
Farag
Amany
Ebeid
Hoda
Elbehery
Mohamed
Abou-Ellail
Random amplified polymorphic DNA (RAPD)-PCR genomic fingerprinting and partial sequencing of the 16S rRNA gene were evaluated on two insects collected from the Egyptian field which could belong to B. hebetor and B. brevicornis to investigate their genetic relatedness and to establish the value of techniques for their identification. Nearly identical RAPD-PCR profiles and identical 467 bp fragments of the 16S rRNA genes indicated many of genetic diversity between the two insects under study. The low levels of similarity (78.21% in the partial 16S rRNA genes and 86% in RAPD-PCR) appeared between the insects B. hebetor egypt and B. brevicornis egypt. However, 16S rRNA genes and RAPD-PCR provided an effective means of differentiating between members of the taxa. Moreover, a phylogenetic tree constructed from 16S rDNA sequences showed that B. hebetor egypt clustered with the B. hebetor with a degree of similarity 92%, but B. brevicornis Egypt clustered in a separated group. However, RAPD-PCR and partial sequencing of the 16S rDNA analysis raises questions about the taxonomic positioning of the two insects isolated from the Egyptian environment.
RAPD-PCR and 16S rRNA genes
bracon species
2013
12
01
99
107
https://eajbsc.journals.ekb.eg/article_16099_36eaf3351f20a217a4d2d94ff89d322c.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
The Potency of Aedes species in transmitting dengue fever virus with evaluating the susceptibility of vector larval stages to some insecticides.
Jazem
Mahyoub
Hamed
Ghramh
KH.
AL-Ghamdi
Nizamuddin
Farooqi
The present study was planned to use the technique of Polymerase chain reaction (PCR) to detect the virus of dengue fever in mosquito females of Aedes aegypti (L.) and Ae. caspius (Pallas). The results indicated that all samples tested for virus of dengue were found negative (-) except one sample of Ae. aegypti which was positive(+). On the other hand, susceptibility levels of mosquito larvae of field strains of Ae. aegypti, which is the vector of dengue fever, to some insecticides currently in use in Jeddah governorate were determined. The LC50 values (concentration which kills 50% of mosquito larvae) were taken in consideration. Mosquito larvae of Ae. aegypti proved to be more susceptible to Bactilarvae (0.012 ppm) than Safroten (0.020 ppm), Temephos (0.032ppm), Solfac (.039 ppm) and Icon (0.047ppm), respectively.
Dengue fever
Aedes aegypti
Ae. caspius
Insecticides
PCR
2013
12
01
109
115
https://eajbsc.journals.ekb.eg/article_16100_089771eae2f7145ddad604d5ba03bec9.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2013
5
2
Determination of Organic Acids in Saudian Bee Honey Types
Nafea
A.
Zidan
W.
Asmaa
Fawzy
Sehata
A.
Eight types of bee honey represented different regions of Saudi Arabia country. Fakhera, Rabie Foyad, El Bashaier, Rabie Kabba, El Saiel Al Kabeer, El Nokhbba, Taba and OmmAzooba were analyzed to determine several organic acid were found as a chemical marker of Saudian bee honey types. It was proved that among the detected acids, formic and Malonic acids were the main acid in the eight honeys, also were detected in the highest amounts as compared with the other organic acids. Oxalic, shikimic, butiric and benzoic acids were not detected in any of the Saudi Arabia tested honeys. Tartaric acid was detected only in Rabie Kabba honey. We can suggested that organic acid profiles can help to distinguish between different honeys, according to their botanical and geographical origins, and can be also used to protect honey spoilage.
bee honey
organic acids
Saudi Arabia
2013
12
01
117
120
https://eajbsc.journals.ekb.eg/article_16101_02bed068321fab02badf95d88ee31960.pdf