2024-03-28T21:19:16Z
https://eajbsc.journals.ekb.eg/?_action=export&rf=summon&issue=2651
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Immunoassay of anti-HCV and seroepidemiological surveillance of hepatitis C virus infection
Sarah
Ahmed
Sabry
El-Sayed
Wajed
Al Shammari
Infection with hepatitis C virus (HCV) is a common global cause of chronic liver disease, which is also true in Saudi Arabia. HCV prevalence in Saudi Arabia varies in different provinces being highest in the Western and Southern provinces. Most of the studies among blood donors documented a decrease in HCV prevalence, probably due to increase awareness and improved socioeconomic status. The present study was designed to investigate the prevalence of hepatitis C infection among general population of Hail region, Saudi Arabia and to determine titer of anti-HCV in patient serum. A total of 100 participants in he study survey with average age of 18-63 years, were preformatted a questionnaire including the demographic data, socio-economic status and medical history. Three mL of venous blood were collected from 37 participants, in a container with strict aseptic precautions. Collected serum samples were used for serological evaluation of anti-HCV infection, by using ELISA. Among the participants in the study survey ,HCV positivity percentage was found to be (1%) (One /100) in our study. Thirty seven serum samples were examined for anti-HCV, thirty of them were positive with titer ranged from (10.512 to 1859.395 IU/ml), while seven samples were negative, In conclusion, the obtained data revealed, low prevalence of HCV in Hail region, Saudi Arabia.
seroprevalence
HCV
anti-HCV
ELISA
hail
2015
12
01
1
9
https://eajbsc.journals.ekb.eg/article_13685_308bf7ecbf6884a2a8886080eeed59c3.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Immunohistochemical IHC Study Expression of Vascular Endothelial Growth Factor (VEGF) and Endostatin in Breast Cancer Groups in Slahaldeen Women Patients
Asmaa
Alwan
Samira
abdullah
Farooq
Mohammad
Twenty Iraqi breast cancer cases were included in this study to detect the protein expression of vascular endothelial growth factor (VEGF) and endostatin (ES) in the women patients from Saladin governorate breast tissues, among breast cancer cases. Hematoxylin and Eosin (H and E) stained then immunohistochemically (IHC) designed for vascular endothelial growth factor (VEGF) and Endostatin. Regarding to histological grading, the results showed that among 20 patients 17 cases were grade II and 3 cases were grade III, while staging system 8 cases were stages (II); 12 cases were stage III. Immunohistochemical IHC study expression of Vascular endothelial growth factor VEGF in breast cancer groups shows positive expression found in 14 (70%) out of 20 cases of the Saladin breast can cercases with P value <0.001, Endostatin expression was observed in 15 (75%) out of 20 samples of the Saladin breast cancer patients with P value <0.05. Correlation between the two studied markers VEGF and ES was studied statistically and the study revealed that there was a strong significant relationship between the VEGF expression and expression of ES (P value <0.01), and also there was significant relationship between expression of VEGF and ES with grading (P value >0.001) and staging system of breast cancer patients (P value >0.05).
IHC
VEGF
ES
2015
12
01
11
17
https://eajbsc.journals.ekb.eg/article_13686_51a7e91686c9a4330a11c40246e6696a.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Anti-Cancer Activity of the Rubus idaeus Extracts Against HepG2 and L20B Cell Lines Using Tissue Culture Technique
Nahla
Assad
Batool
Dheeba
Farooq
Mohammad
Abdullah
Hamad
The extracts were prepared from ripe blackberries. The extracts were tested against the human hepatocellular carcinoma cell line, HepG2, and the mouse cell line L20B using a neutral red assay. The extracts showed a clear inhibition rate, in a dose dependent manner in both cell lines. In addition, the inhibition rate was significantly higher (P < 0.05) for alcoholic extracts in both cell lines compared to aqueous extracts. The results clearly show that Rubus idaeus fruit extracts have potent cytotoxic and cytostatic effects on both cell lines. On the basis of these results, we can conclude that Rubus idaeus has a strong cytotoxic effect on both human and mouse cancerous cells.
Rubus idaeus
Cytotoxic activity anti-cancer HepG2 L20B
2015
12
01
19
23
https://eajbsc.journals.ekb.eg/article_13687_d139f4779c69a30df3e1c9c289b3bb8e.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Growth and Physiological Responses of Wheat Seedlings to Cadmium Alone and in Combination with SiO2 Nanoparticles
Javad
Karimi
Sasan
Mohsenzadeh
Heavy metals are the major environmental pollutants, mainly in areas with high anthropogenic activities. In this study, the effects of three cadmium (Cd) concentrations (30, 60, and 120 mg-1l) alone and in combination with two concentrations of SiO2 nanoparticles (10 and 100 mg-1l) on growth and some physiological parameters of wheat (Triticum aestivum) seedlings were investigated. Cadmium treatments decreased the fresh and dry weight of roots, shoots, chlorophyll, carotenoid and total protein contents of the leaf tissues significantly. It also increased the amount of proline, lipid peroxidation and catalase activity of wheat seedling. The toxic effects of cadmium ions on growth and physiological activities of wheat seedlings were reduced in the presence of SiO2 nanoparticles.
Cadmium toxicities
wheat seedlings
SiO2 nanoparticles
alleviating effects
2015
12
01
25
39
https://eajbsc.journals.ekb.eg/article_13688_cde24e262c578193d81262a7ff8dacfc.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Phytosynthesis of Gold Nanoparticles Using Leaf Extract of Sedum pachyphyllum
Javad
Karimi
Sasan
Mohsenzadeh
The developments of rapid, simple, cost-effective and eco-friendly procedures for the synthesis of nanoparticles are very important in the field of nanotechnology. The present study deals with the green synthesis of gold nanoparticles using leaf extract of Sedum pachyphyllum as the reducing agent. The synthesis is carried out at room temperature in the laboratory ambient. Merely, few minutes were necessary for the synthesis of gold nanoparticles. Exposed to leaf extract, the aqueous gold ions were reduced and resulted in gold nanoparticles. The formation of gold nanoparticles was confirmed in the presence of an absorption peak at 556 nm using UV–visible spectrophotometer. Field Emission Scanning Electron Microscopy (FESEM) analysis showed gold nanoparticles, which are about 80 mm. Fourier Transform Infrared Spectroscopy (FTIR) revealed possible involvement of reductive groups on the surfaces of gold nanoparticles. Stable gold nanoparticles were formed by treating an aqueous solution of HAuCl4 with the plant leaf extracts as reducing agent of Au3+ to an Au0. This method provides a safe and green synthesis comparable to more chemical methods and can be used in areas such as medical, foods and cosmetics.
Phytosynthesis
Gold nanoparticle
leaf extract
Sedum pachyphyllum
2015
12
01
41
46
https://eajbsc.journals.ekb.eg/article_13689_9e41e019047d7cc4b60ec3f08a40e058.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Molecular Identification of Aspergillus flavus Using RAPD Markers
Sanaa
Mohamad
The aim of this study is to isolate and identify A. flavus and study the genetic diversity among these isolates by using RAPD. Eleven collected samples were characterized depending on its morphological state, then DNA was extracted from them. RAPD markers are randomly banding with sites of genome more than, markers, where the primer UBC 809achieved discriminative power (19.1) and 43 bands, while6 achieved discriminative power (17.1) with 32 bands. There were more efficiency in specific binding, then RAPD primers have great binding to produce unique band, when 9 primers from 10 primers, 9 produced (5) unique bands, while RAPD markers showed low ability to produce unique bands, 3primers from 9 primers were produced as unique bands. The dendrogram of RAPD was reverted than isolates number 5 and 7 which had the genetic diversity 0.33361, while the isolates number 5 and 6 had the lowest genetic similarity 0.98521 in contrast with markers which showed isolates number1 and 2 genetic diversity 0.97826 while the isolates number 5 and 7 had the lowest genetic similarity 0.10253.
Molecular
identification
Aspergillus flavus
RAPD
2015
12
01
47
54
https://eajbsc.journals.ekb.eg/article_13690_867651b18cca541196f6d2b767db6c13.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Biomonitoring Of Some Heavy Metals In Water Of El Salam Canal using Oreochromis niloticus As Bioindicator
Amira
Aly
El-Sayed
El-Sayed
This study was initiated to assess the levels of (Fe, Cu, Cd, Pb, Zn, Co, Mn and Ni) in water samples and Oreochromis niloticus tissues (muscles, gills and liver) collected from Fum El-Salam canal, Hadous drain and mixing point of El-Salam canal with Hadous drain throughout Summer 2014. Levels of physicochemical parameters of water samples (TDS, EC and ammonia) were significantly high (P<0.05) in Hadous drain and mixing point of El-Salam canal with Hadous than the permissible levels of the law 48/1982. The results showed that Zn, Fe, Mn and Cu recorded 1.612±0.6-<0.001-0.011±0.001,0.56±0.29- 0.479 ± 0.03- 0.075±0.01, 0.25±0.028 - 0.179±0.01 - 0.046±0.003, 0.045± 0.01 - 0.019±0.01 - 0.009±0.007 mg/l in Hadous drain, Mixing point and Fum El-Salam canal respectively, while the increase in Zn and Mn for Hadous drainage exceeded the permissible values sets by USEPA (1996). The discharging of Bahr Hadous drainage water to El-Salam canal leads to alteration in water quality of El-Salam canal as it increases heavy metals loads which implicate pollution, such deterioration showed in the studied tissues of Oreochromis niloticus. It is recommended to treat the different wastes before discharging to El-Salam canal.
El-Salam Canal
Hadous drain
Heavy metals
O. niloticus
2015
12
01
55
68
https://eajbsc.journals.ekb.eg/article_13691_15486549420eb383d3e072beac664457.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Association Analysis of Chronic Kidney Disease and Its Risk Factors for Hail Region Population
Abdelbaset
Elasbali
Hypertension and diabetes mellitus are important risk factors for chronic kidney disease (CKD). The purpose of the present study was to identify genetic variants that confer susceptibility to CKD in individuals with or without hypertension or diabetes mellitus, thereby contributing to the personalized prevention of CKD in such individuals separately. The study population comprised 299 unrelated individuals, including 176 subjects with CKD and 172 controls. The 75 polymorphisms were selected by genome-wide association studies of chronic kidney disease and hypertension with the use of the Gene Chip Human Mapping 500K Array Set (Affymetrix). The genotypes for these polymorphisms were determined by a method that combines polymerase chain reaction and sequence-specific oligonucleotide probes with suspension array technology. The χ2 test, multivariable logistic regression analysis with adjustment for covariates, as well as a stepwise forward selection procedure revealed that two different polymorphisms were significantly (P<0.005) associated with the prevalence of CKD in individuals with or without hypertension or diabetes mellitus: the A→G (Lys625Arg) polymorphism of CDH4 (rs6142884) in individuals without diabetes mellitus, and the C→T polymorphism of PTPRN2 (rs1638021) in individuals with hypertension and diabetes mellitus. No polymorphism was significantly associated with CKD in individuals with or without hypertension, in those with diabetes mellitus, or in those without hypertension or diabetes mellitus. Stratification of subjects based on hypertension or diabetes mellitus may thus be fundamental to achieving the personalized prevention of CKD with the use of genetic information.This preliminary study provided information on the frequency of CKD and its associated risk factors in the Hail region. However, larger population needs to be studied to establish the role of these risk factors in the etiology of CKD in Hail region.
hail
Genetics
Polymorphism
Chronic Kidney Disease
Diabetes mellitus
Hypertension
2015
12
01
69
79
https://eajbsc.journals.ekb.eg/article_13692_3a06e93eb9815accd349f40324bdb541.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
The Consciousness of The Human Precise to Plenty Food among Women and Children from low income group (Slum Region)
Eyad
Al-Shammari
Mohd
Saeed
Samra
Siddiqui
Israr
Ahmad
First-rate nutrition is vital not only for human health but also for national economic and social development. The objective of this review is to assess the realization of the right to adequate food focusing on women and children living in the slums of India. The review categorizes the realization of the right into two classes: the right holders (mothers or caretakers and their children) and duty bearers (peoples/Individuals responsible for providing the necessary facilities as per the rights of the right holders). Conclusions were drawn regarding the following aspects: nutritional status of selected right holders, availability and accessibility of foods, existence of nutritional related health and sanitation facilities and whether appropriate legal, regulatory and institutional frameworks necessary to realize right to food are in place.
Human health
National economic
Slum
2015
12
01
71
87
https://eajbsc.journals.ekb.eg/article_13693_0e1d0077c7dee51a20ab2fcf9478ad75.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Bioinformatic Analysis of the Beauvericin Gene from Beauveria bassiana and Insecticidal Effect on Spodoptera littoralis (Boisd)
Sahar
Ali
Yasmin
El-sayed
Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activitie. Total DNA was extracted from B. bassiana Egyptian isolate. The integrity of total DNA was estimated by ethidium bromide re, taq DNA polymerase and (Forward and Reverse) primers directly which designed to amplify the beauvericin gene. The nucleotide sequence of the PCR-amplified fragment for the beauvericin gene of B. bassiana EG-isolate was done to determine the relationship with other B. bassiana isolates registered in Gen Bank, and was aligned by using DNAMAN program (Wisconsin, Madison, USA) with another three B. bassiana isolates. The predict numbers of amino acids were produced from translation of beauvericin gene nucleotide sequence were 211 amino acids. A phylogenetic tree of beauvericin from B. bassiana Eg. isolate revealed 100% a high degree of similarity to beauvericin of B. bassiana non ribosomal cyclo depsipeptide synthetase, (Accession no. AC130655), 94.3% and 94.8% to beauvericin of B. bassiana biosynthetic protein (Accession no. ADO 60131) and beauvericin of B. bassiana biosynthetic protein, (Accession no. AFJ44691) respectively. While, the insecticidal effect of toxins crude extraction was studied in this wake . Toxins crude extraction due to isolate of B. bassiana investigated against 3rd inster larvae of S. littoralis. After 4 days, the percentage of mortality were 51.00%, 57.50%, 79.00% and 96.50% in the concentrations 25, 50, 75 and 100%, respectively.
Bioinformatic
the beauvericin gene
Beauveria bassiana Spodoptera littoralis (Boisd)
2015
12
01
89
99
https://eajbsc.journals.ekb.eg/article_13694_4043885a285f1be323f42fb076608aba.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Ameliorative Effect of Oregano Essential Oil on Mycotoxins-Induced Immune Impairments in Growing Japanese Quail
Heba
Abdelrazek
Mohamed
Yusuf
Marwa
Hassan
Mohamed T
Soliman
Adel
El Nabtiti
Mycotoxins contaminated feed is one of the most serious problems in poultry and animal production sectors. Therefore, the current study aimed to investigate the effects of dietary oregano essential oil (OEO) for 4 weeks against the adverse effect of aflatoxins (AFs) on immunity, oxidative stress, Total and differential leucocytes counts (TLC and DLC), interleukin 1 (IL-1), nitric oxide (NO), lysozyme enzyme activity, serum malondialdehyde (MDA), catalase, superoxide dismutase (SOD) and reduced glutathione (GSH). Beside growth performance [Weekly body weight gain and feed conversion ratio (FCR)] were also determined. A total of 132 male, 2 weeks old Japanese quails chicks (Coturnix japonica) were used in this study and equally divided into 4 groups. Group I is considered as negative control at which birds fed only basal diet, group II served as positive control and were fed AFs 2.5 mg /Kg supplemented basal diet, Groups III and IV were fed basal diet with 2.5 mg AFs /Kg and treated with OEO at a rate of 200 and 400 mg/Kg , respectively. Regarding growth performance, there was a significant (P<0.05) reduction in weight gain and FCR at 4th week of the experiment in group II, additionally different pattern was noticed in group IV which represented a significant (P<0.05) improvement as compared to the other AFs treated groups and was nearly similar to that of group I. Concerning Lipid peroxidation, oxidative stress enzyme and immune parameters at 4th week of the experiment, MDA was significantly (P<0.05) increased in AFs treated quails (II and III) but when AFs co-administered with 400 mg OEO, MDA was nearly similar to that of group I. The SOD, catalase, and GSH were decreased significantly (P<0.05) in groups (II and III) and was remarkably improved in group IV. The NO level was increased significantly (P<0.05) while lysozyme activity was decreased significantly (P<0.05) in group II, while they were decreased and increased in group IV, respectively. IL-1 and globulin as well as TLC were declined significantly (P<0.05) in groups II and III and improved nearly similar to control one in group IV. In conclusion, OEO has a potential and a protective effect against AFs B1 immuno-deteriorating effect in Japanese quails especially at 400 mg/ kg dose.
Aflatoxins
Oregano oil
immunity
quails
2015
12
01
101
114
https://eajbsc.journals.ekb.eg/article_13695_d810e792774d9aa855730420c9d7d927.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
Biochemical Effects of Bradykinin Potentiating Factor (BPF) Isolated from Scorpion Venom ( Leiurus quinquestriatus) against CCl4- Liver Injury in Male Albino Rats.
Muhammad
Salman
The main purpose of this study is to evaluate the ability of bradykinin potentiating factor (BPF) isolated from scorpion venom (Leiurus quinquestriatus) in treatment of liver injuries which induced by injection of CCl4in male Albino rats. Male Albino rats (250±20 g. body weight) were divided into four groups. In the control group; Albino rats were interaperitoneally (i.p) injected with 100 µL saline solution. The second group (i.p) injected with BPF in 100 µL saline solutions (1μgm/g. b. w. per 5 days). Third and fourth groups were i.p. injected with 0.5 ml/kg body weight (b. w.) twice weekly of CCl4 for fifteen days, after that only the fourth group was treated by BPF in 100 µL saline solutions (1μgm/g. b. w. per 5 days). The results indicated that, CCl4 injection induced a significant decrease in serum catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), total protein and albumin, within thirty days post-injection of CCl4 as compared to the normal control group. In contrast, CCl4 induced a significant increase in malondialdehyde(MDA), aspartate amino transferase (AST), alanine amino transferase (ALT), and alkaline phosphatase (ALP) compared to normal control animals. The efficiency of BPF treatment is alleviation the effects of CCl4 on these parameters. The improvement of these parameters may be attributed to the release antioxidant and cytokines and/or amelioration of the toxic effects of CCl4 on the liver.
Scorpion venom
L. quinquestriatus
BPF
CCl4
liver injury and
albino rats
2015
12
01
115
126
https://eajbsc.journals.ekb.eg/article_13696_1443be26b61352bdfcf1ca30c46e30b1.pdf
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-0767
2015
7
2
RAPD-Based Genetic Variances Between Female and Male Adult House Fly Musca domestica L.1758 (Diptera, Muscidae) From Qena, Egypt
Mohammed
EL-Mahdi
The genomic DNA from female and male adults house fly Musca domestica L. was subjected to theRAPD analysis using a set of random primers. A ten (10) selected primers produced a distinctive RAPD fingerprint of various bands ranging in size from 350 to 1700 bp. A total of 57 RAPD bands/loci were produced with an average of 5.7 bands per primer, from them 18 bands with a level of monomorphism of 31.58% were common between female and male, and 39 were polymorphic corresponding to a level of polymorphism of 68.42 %. Both female and male genotypes displayed high band frequencies, however male showed to be slightly higher than female ones. Result of this study suggested occurrence of gender-based internal genetic variations and heterozygosity of common house fly adults Musca domestica L. that is slightly higher in males than females. This would be necessitated for the species survival, successful environmental adaptations and resistance to chemical applications.
Genetic variances
RAPD-PCR
genotype
Diptera
Musca domestica
Qena
2015
12
01
127
134
https://eajbsc.journals.ekb.eg/article_13697_cd217cf0a288323ccc07bb2a1ae081cd.pdf