Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Effect of Selenium and Bee Pollen Against Immunotoxicity and Hepatotoxicity Induced by Cadmium in Male Albino Rats.
1
19
EN
Abd-El Reheem
A.
El Shater
Department of Zoology, Faculty of Science, South Valley University
Rana
A.
Ali
Department of Zoology, Faculty of Science, South Valley University
rana.ahmed@svu.edu.eg
Ahlam
F.
R
Department of Zoology, Faculty of Science, South Valley University
10.21608/eajbsc.2019.28688
Cadmium is a toxic naturally occurring element that affects numerous organ systems in humans, the present study was designed to study the role of bee pollen and selenium against cadmium chloride induced immunotoxicity and Hepatotoxicity in male albino rats.<br /> Forty (40) male albino rats were used for the experiment. Group I was intraperitoneal (i.p.) Injected with saline solution, G2 (i.p.) Injected with CdCl2 (1 mg/kg b.w.), G3 were (i.p.) Injected with CdCl2 (1 mg/kg b.w.) and then receive selenium (i.p.) Injection at a dose of (0.5 mg/kg/day). G4 (i.p.) Injected with CdCl2 (1 mg/kg b.w. day by day) and then received bee pollen orally at a dose of (10 g/kg/day). Animals were exposed to treatment once daily for 10weeks. After the last day of treatment, animals were sacrificed and blood samples collected for hematological analysis and serum separated for serum liver enzymes analysis and the liver separated for oxidative stress analysis. Results obtained in this present study revealed a significant increased at (p>0.05) in WBCs, Monocyte , Lymphocyte , Granulocyte and The activities of IgG and IgM, aspartate aminotransferase (AST), alanine transferase (ALT) ,alkaline phosphatase (ALP), (Total- Direct) bilirubin and Malondialdehyde (MAD), while showed a significant decreased at (p>0.05) in albumin and protein, Glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT) in group treated with CdCl2 only when compared with normal group. However, Effects of bee pollen and selenium on immunotoxicity and hepatic injury due to cadmium-induced oxidative stress revealed a significant decreased at (p>0.05) in WBCs, Monocyte, Granulocyte and The activities of IgG except Lymphocyte and IgM are not affected also aspartate aminotransferase(AST), alanine transferase (ALT) ,alkaline phosphatase (ALP), (Total- Direct) bilirubin and Malondialdehyde (MAD) showed a significant decreased at (p>0.05), while Total protein, Albumin, Glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT) showed a significant increased at (p>0.05) when compared with control group. It is concluded that the bee pollen and selenium have antioxidant properties
cadmium,bee pollen and selenium,Immunotoxicity,Hepatic Injury,Oxidative Stress,Rats
https://eajbsc.journals.ekb.eg/article_28688.html
https://eajbsc.journals.ekb.eg/article_28688_31da92d4c8ad2d82a1a8ab2aada09e88.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
In-Silico Study of Olive’s (Olea europaea L) Bioactive Compounds as Anti-Cancer Agents
21
29
EN
S.
M.A.
Shahida
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
2007.sma@gmail.com
Mohammad
Kuddusa
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Mohd
Adnan
Kausara
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Basil
E. K.
Alshammari
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Musaabc
A. S.
Althaqafi
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Rakan
E. K.
Alshammari
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Sultan
N. H.
Alshmmary
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Hussain.
K.A.
Almudayni
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Khalid.
M.
Alshammary
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Sulaiman
S.
Alsamaan
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Abdalaziz
S.
alduhaim
Department of Biochemistry, College of Medicine, University of Hail, P.O. Box 2440, KSA
Mohd
Saleem
Department of Microbiology College of Medicine, University of Hail, P.O. Box 2440, KSA
Mohammad
Ahmed
Qumani
Department of Pharmacology, College of Medicine, University of Hail, P.O. Box 2440, KSA
10.21608/eajbsc.2019.29079
Recent studies suggest that Olives bioactive compounds are very important and mainly associated with cancer fight. They can suppress tumor growth through the direct interaction with several important proteins which are directly associated with tumor associated pathways such as P53, P16, P21, BCL2, BAX, MDM2, MMP2 and MMP9 proteins. The main aim of the present work is to perform a docking analysis of the Olives bioactive compounds with tumor-associated proteins. To do that, we first retrieved all the known structures of Olive bioactive compounds using different available online databases. Next, we analyzed these structures using Molegro Virtual Docker (MVD-2010, 4.2.0) software. Further, we narrowed four important compounds (namely, Deacetoxyoleuropein aglycone, Hydroxytyrosol, Tyrosol and Erlotinib) of Olive bioactive compounds that showed very distinguished properties. The ADME properties of these four compounds were predicted based on Lipinski’s rule of five. The active sites of the very widely known tumor-associated proteins (P53, P16, P21, BCL2, BAX, MDM2, MMP2 and MMP9) were explored using MVD software. Finally, the active sites of these proteins were docked with the above mentioned four compounds. Based on the docking study, it was concluded that Deacetoxyoleuropein Anglican showed strong inhibition with EGFR, VEGFR1 and VEGFR2.
olive,Cancer,docking,MVD,Therapeutic
https://eajbsc.journals.ekb.eg/article_29079.html
https://eajbsc.journals.ekb.eg/article_29079_648255f8c53cd96de1db05092e097f70.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
The Prevalence of Obesity and the Relationship of Food Intake in the Body Weight of Medical Students of Hail University – Northern Saudi Arabia
31
36
EN
Abdelhafiz
Ibrahim
Bashir
College of Medicine – University of Hail – Saudi Arabia
Abdulrahman
Hasan
Althagafi
College of Medicine – University of Hail – Saudi Arabia
Mohammad
Abdulkarim
Alduheim
College of Medicine – University of Hail – Saudi Arabia
Abdullah
Mohammed
Alshaikhi,
College of Medicine – University of Hail – Saudi Arabia
Naif
Osaywid
Eid alrashede,
College of Medicine – University of Hail – Saudi Arabia
Mohannad
Nasser
Alqwayee
College of Medicine – University of Hail – Saudi Arabia
Ibrahim
Abdelmageed
Ginawi
College of Medicine – University of Hail – Saudi Arabia
Tarig
A. N.
Ginawi
College of Medicine – University of Hail – Saudi Arabia
S.
M.A.
Shahid
College of Medicine – University of Hail – Saudi Arabia
Mohammed
Qumani
Ahmed
College of Medicine – University of Hail – Saudi Arabia
Mohd
Adnan
Kausar
College of Medicine – University of Hail – Saudi Arabia
adnankausar1@gmail.com
10.21608/eajbsc.2019.30334
<strong>Background:</strong> Obesity is becoming major problems in developed as well as developing countries. Obesity is the major cause of a large number of debilitating and life-threatening diseases such as cardiovascular, metabolic and other non-communicable diseases. The Objectives of this study is to estimate the prevalence of obesity among medical students and the role of food intake among medical students. <strong>Methods:</strong> A descriptive cross-sectional design was used. 150 Saudi medical students studying at Colleges of Medicine, Dentistry, Pharmacy, Nursing, and applied medical science in Hail university were invited. <strong>Results: </strong>The mean age of the study population was 33.5 ± 18.7 years with 33.6 ± 19.2 for males and 32.3 ± 16.9 for females. Males to female’s ratio was 1.00:1.01. The overall prevalence of obesity was 64.7%. <strong>Conclusions:</strong> The study showed that the prevalence of obesity among the medical students of Hail University is high, which is comparable to the other studies. This indicates the need to encourage a healthy lifestyle, healthy food habits, and a physically active daily routine, among medical students.
Obesity,Diet Body Mass Index,overweight
https://eajbsc.journals.ekb.eg/article_30334.html
https://eajbsc.journals.ekb.eg/article_30334_4ac9ee81094b194ab4755611a7e16b08.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Genetic Variation among Five Egyptian Clover Cultivars Using Random Amplified Polymorphic DNA (RAPD) and Sequence-Related Amplified Polymorphism (SRAP) Molecular Markers
37
45
EN
Sawsan
S.
Youssef
Department of Genetics, Faculty of Agriculture Cairo University, 12613, Giza- Egypt
Sanaa
A.
Ibrahim
Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt.
Amany
M.
Abdel Azim
Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt.
Etr
H. K.
Khashaba
Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt.
ater_hk@yahoo.com
Ahmed
M.
El-Sharkawy
Department of Genetics, Faculty of Agriculture Cairo University, 12613, Giza- Egypt
10.21608/eajbsc.2019.31758
To estimate the genetic variation among five Egyptian clover cultivars, Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were employed. Sixteen RAPD and six SRAP primers were used in the present study, a total of 34 and 12 bands showed 24.8 and 60% polymorphism percentage. The highest number of RAPD bands was recorded for primers OPE-B-07 (13 bands), followed by OPE-K-04 (12 bands), while the lowest was scored for OPE-B-05 (5 bands). For the SRAP Primers Me1-em2, Me3-em2 and Me3-em4 recorded the highest number of bands (four bands), followed by three bands for Me1-em1and Me3-em3, primer Me3-em1 recorded the lowest band number (two bands only). The dendrogram built based on combined data from RAPD and SRAP analyses clarify the genetic distances among the five Egyptian clover cultivars.
Egyptian clover – RAPD – SRAP – molecular markers
https://eajbsc.journals.ekb.eg/article_31758.html
https://eajbsc.journals.ekb.eg/article_31758_bbd307bdc4cb4109abb5e562939e64c8.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Reduction in the Formation of Micronucleated Polychromatic Erythrocytes Induced by Cisplatin in Bone Marrow Cells of Rats by using Antioxidants.
47
55
EN
Fawzyea
Abdelhady
Zaied
Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Sabha
Elsayed
Elballat
Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
sabhael@zu.edu.eg
10.21608/eajbsc.2019.33962
<span>Cisplatin is extensively used as a main chemotherapeutic drug for the treatment of many types of tumors. Cisplatin exerts excellent anticancer action, but its clinical use is frequently limited due to the development of severe side effects in the treated patients such as nephrotoxicity, anemia and bone marrow damage. Cytotoxicity of cisplatin is closely related to the generation of reactive oxygen species leading to oxidation of vital components of the cell (DNA) and genomic instability. <strong>Results and Conclusion:</strong> The results supported that the combination between curcumin and vitamin C (with more adjusted dose of vitamin C) exerted the best ameliorative effect against the cisplatin induction of micronucleated polychromatic erythrocytes. </span>
Cisplatin,vitamin c,Curcumin,Micronucleated polychromatic erythrocytes,Micronucleus test,antioxidants
https://eajbsc.journals.ekb.eg/article_33962.html
https://eajbsc.journals.ekb.eg/article_33962_7a9b14a0358ad440894d298d0396f862.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Constitutive Heterochromatin Pattern of Five Domestic Duck Breeds, (Aves: Anatidae) in Egypt
57
69
EN
Abdeltawab
M. Ata
Ata
Department of Genetics, Faculty of Agriculture, Minia University, 61519 El Minia, Egypt
Hassan
Z.
Allam
Department of Genetics, Faculty of Agriculture, Minia University, 61519 El Minia, Egypt
Ahmed
E.
Abousalha
Department of Genetics, Faculty of Agriculture, Minia University, 61519 El Minia, Egypt
Walid
M.
Fandy
Department of Genetics, Faculty of Agriculture, Minia University, 61519 El Minia, Egypt
Anwaar
S. M.
Abu Shnaf
Department of Zoology, Faculty of Science, Minia University, 61519 El Minia, Egypt
anwaarsalama78@yahoo.com
10.21608/eajbsc.2019.36903
Constitutive heterochromatin patterns of five domestic duck breeds three of <em>Anas platyrhynchos</em> (Dumyati, Khample and Pekin) and two of <em>Cairina moschata</em> (Muscovy and Sudani) occurring in Egypt have been described. Results indicated that although the constitutive heterochromatin (C-banding) pattern of three <em>Anas platyrhynchos </em>breeds shared the same distribution of heterochromatin there was an obvious variation in heterochromatin size. In addition, the C-banding patterns of the two <em>Cairina moschata</em> breeds (Muscovy and Sudani) were approximately similar. There wasvariation in size, number and occurrence of C-band blocks on micro-chromosomes between the examined five duck breeds. This may be attributed to the variation of euchromatin content and its correlation with chromosome size and arrangement of constitutive heterochromatin. Furthermore, it may be due to genome mixing through hybridization between related species and/or genera. The present results have shown that discrimination between <em>Anas platyrhynchos</em> (Dumyati, Khample and Pekin) and <em>Cairina moschata</em> (Muscovy and Sudani) duck breeds could be realized via cytogenetical markers such as presence of entirely heterochromatic chromosome pair no.8 in breeds belong <em>Anas platyrhynchos</em> whereas it was euchromatic in breeds of <em>Cairina moschata</em>. In addition to chromosome pair no.8, C-banding pattern of autosome pair no.3 and sex chromosome Z Could discriminate between the duck breeds of <em>Anas platyrhynchos</em> and <em>Cairina moschata</em>. Moreover, the constitutive heterochromatin amounts have significantly varied between the ducks belonging to the two genera. Thus, about only one-third of the micro-chromosomes in the three <em>Anas</em> breeds, exhibited large and visible C-banding blocks in both males and females while up to half of them in the two <em>Cairina</em> breeds. Hence, the duck breeds common in Egypt could be distinguished from others occurring elsewhere by C-banding pattern but this will be confirmed in further biochemical and molecular studies (under publication).
Constitutive heterochromatin,C-banding,Duck,Anatidae,Egypt
https://eajbsc.journals.ekb.eg/article_36903.html
https://eajbsc.journals.ekb.eg/article_36903_15a96ff635ee23a85d038f8436f38da5.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Prevalence and Severity of Anemia in CKD patients
71
83
EN
Raed
Alharbi
Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Kingdom of Saudi Arabia
ralharbi@bu.edu.sa
10.21608/eajbsc.2019.39109
<strong>Background</strong>:Anemia is one of the well-recognized and significant complications of chronic kidney disease (CKD) which manifests noteworthy association with the progression of CKD, the inevitable necessity for blood transfusion, prolonged hospitalization. This study aimed to conduct a comprehensive study as regards the prevalence of anemia and its association with laboratory parameters in CKD patients.<br /> <strong>Methods:</strong>The Chronic Kidney Disease-Epidemiology Collaboration (CKD-EPI) equation was applied to calculate the estimated glomerular filtration rate (eGFR) and the patients with hemoglobin (Hb) level < 13.0 g/dL in case of males and that < 12.0 g/dL in females was defined as anemic. The patients were stratified according to their Hb level into four different categories: Hb 12 g/dL.<br /> <strong>Results:</strong>Demographic status of CKD patients: Male/Female = 106/123 (M/F% = 46.28/53.71) with mean age 54.94 ± 17.98 and 55.13 ± 14.27, respectively. Out of 229 CKD patients, 157 (69%) were anemic, and 72 (31%) patients were non-anemic. The mean eGFR of anemic and non-anemic CKD patients was 10.14 ±13.57 and35.74 ± 30.36 with a significance level <strong>(</strong><em>P</em><0.001<strong>)</strong>. Anemia was more prevalent in females’ (36%) than males’ patients (32%). The Prevalence for Hb < 12 g/dL for male and female patients was 55.29% and 79.25%, respectively, while that < 11 g/dL for male and female was found to be 39.03% and 63.21%.<br /> <strong>Conclusion:</strong>The prevalence of anemia in CKD population is notable and correlates with the progression of CKD
Anemia,CKD,eGFR,prevalence
https://eajbsc.journals.ekb.eg/article_39109.html
https://eajbsc.journals.ekb.eg/article_39109_b1a162642d37f3a403ec4a875dd30f3f.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Potential Anti-Inflammatory Effects of the Egyptian Scorpion (Androctonus amoreuxi) Venom in Rheumatoid Rat Model
85
102
EN
Ahmad
k.
Hassan
Zoology Department, Faculty of Science, Port Said University, Egypt
ahassan@sci.psu.edu.eg
Eslam
M.
Elfeky
Clinical Pathology Department, Manzala General Hospital, Dakahlya, Egypt
Osama
A.
Abbas
Zoology Department, Faculty of Science, Port Said University, Egypt
Mohamed
A.
Hefny
Rheumatology Department, Faculty of Medicine, Suez Canal University, Egypt.
10.21608/eajbsc.2019.42306
Rheumatoid arthritis (RA) is a systemic autoimmune disorder, which takes place in the synovial tissues. This study aims to assess the possibility of using the Egyptian scorpion <em>Androctonus amoreuxi</em> venom (SV) on the treatment and management of RA. The venom was collected from scorpions using electrical stimulation then processed to get crude which was subcutaneously injected to arthritis induced rats. RA was induced in the rats by a single injection of 0.1 ml of Complete Freund's Adjuvant (CFA) at the footpad of the right hind paw of 32 adult male albino rats. Animals were divided into five groups, normal control, RA none treated, SV treated with low and high doses, and methotoxate treated. Results exhibited that SV (0.24 and 0.48 mg/kg) treatment relived pain, improved movement and reduced paw edema and joint swelling volume in arthritic rats. SV had diminished tissue damage in which malondialdehyde (MDA), lactate dehydrogenase (LDH), and creatine kinase (CK) were significantly decreased compared to arthritic rats without side effects in liver, blood, and kidney parameters. Histological examination showed marked improvement in the joint structure after SV treatment. Immunohistochemical staining showed a significant decrease in the expression of nuclear factor-kappa B Cell 65(NFкB-p65), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the arthritic joint after treatment with SV. In conclusion, SV possesses therapeutic properties in RA treatment and management due to its analgesic, antioxidant, and anti-inflammatory effects without noticeable side effects.
Scorpion venom,CFA,Rheumatoid Arthritis,Anti-inflammatory
https://eajbsc.journals.ekb.eg/article_42306.html
https://eajbsc.journals.ekb.eg/article_42306_f66cab2694796702fcdb415260f1583d.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Molecular Changes in Salivary Glands of Culex pipiens pipiens (Diptera: Culicidae) at Different Feeding Stages
103
110
EN
AlaaEddeen
M.
Seufi
-Department of Basic Sciences, Deanship of Common First Year, Jouf University, KSA.
-Department of Entomology, Faculty of Science, Cairo University, Egypt
alaaseufi@yahoo.com
Ismail
E.
I.
Department of Entomology, Faculty of Science, Cairo University, Egypt.
Soad
A.
El-kenawi
Department of Entomology, Faculty of Science, Cairo University, Egypt.
10.21608/eajbsc.2019.42993
This investigation was conducted to detect genetic variations between male and female <em>Cx. pipiens pipiens</em> during different feeding stages using DD-PCR technique. Salivary glands were collected at 3, 12, 24, 48, 72 h post-sugar administration for both males and females, at 3, 12, 24, 48, 72 h post-blood meal, and post-oviposition for females. Unfed, starved and sugar-fed individuals were used as controls. DD-PCR technique was employed using 4 arbitrary primers separately. Several common bands were detected in all samples. Other hands were stage-specific. Many bands were up-regulated or down-regulated at different feeding stages. Our results showed the powerfulness of DD-PCR to discover changes of gene expression in salivary glands of male and female <em>Cx. pipiens</em> <em>pipiens</em> at different feeding stages. Sequencing of the differentially displayed bands would be very useful in the identification of the genes involved in the feeding process of mosquitoes.
Salivary glands,DD-PCR,Culex pipiens,feeding stages,blood-feeding
https://eajbsc.journals.ekb.eg/article_42993.html
https://eajbsc.journals.ekb.eg/article_42993_2924ebff55f4a7bfe0315a8117671758.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Evaluation of three Glomerular Filtration Rate (GFR) Equations Discriminates Stages of Chronic Kidney Disease (CKD) in Sudanese Patients
111
122
EN
Tarig
A.N
Ginawi
1-Department of Biochemistry, College of Medicine, University of Hail, Kingdom of Saudi Arabia
2-Department of Biochemistry, College of Medicine, Alzaiem Alazhari University, Khartoum Bahri, Sudan
Siham
M.A.
Bakhit
Department of Biochemistry, College of Medicine, Alzaiem Alazhari University, Khartoum Bahri, Sudan
Mohd
Adnan
Kausar
Department of Biochemistry, College of Medicine, University of Hail, Kingdom of Saudi Arabia
adnankausar1@gmail.com
10.21608/eajbsc.2019.43564
<strong>Background: </strong>Chronic kidney disease (CKD), represent a diagnostic challenge. CKD is an asymptomatic disease, tests for screening and early diagnoses are critical in nephrology.<br /> <strong>Objectives: </strong>To assess the potential role of a three glomerular filtration rate (GFR) calculation equations in discriminating between stages of chronic kidney disease (CKD) in Sudanese patients.<br /> <strong>Methods: </strong>The study was carried out at Khartoum State, Sudan from July 2017 to August 2017. Three hundred and seventy-five (375) CKD patients aged 18–90, their GFR have been estimated by three equations as follows: combined creatinine (cr) and cystatin C (cysC) equation (GFRcr-cysC), Modification of Diet in Renal Disease (MDRD) equation (GFRcr) and CystatinC-based equation (GFRcysC). The discriminating ability between stages was tested by ROC analysis.<br /> <strong>Results:</strong> The area under the receiver operating (AUC) curve values of the discriminated stages was tested by ROC analysis as the follows: between stage 1 and 2 were: GFRcr-cysC; 0.98 (p > 0.001), GFRcr; 0.77 (p < 0.001) and GFRcysC; 0.82 (p < 0.01). Between stage 2 and 3 were: GFRcr-cysC; 0.98 (p > 0. 001), GFR cr; 0.82 (p < 0.001) and GFRcysC; 0.89 (p < 0. 001). Between stage 3 and 4 were: GFRcr-cysC; 0.96 (p > 0. 001), GFRcr; 0.92 (p < 0.001) and GFRcysC; 0.67 (p < 0. 001). Between stage 4 and 5 were: GFRcr-cys; 0.99 (p > 0. 001), GFRcr; 0.95 (p < 0.001) and GFRcysC; 0.65 (p < 0. 001). GFRcr-cysC had the highest AUC of all.<br /> <strong>Conclusion:</strong> Taking together, the study demonstrated that GFRcr-cysC equation is a valuable marker for early detection of CKD
Plasma cystatin C,Plasma creatinine,Glomerular Filtration Rate,CKD,ROC analysis
https://eajbsc.journals.ekb.eg/article_43564.html
https://eajbsc.journals.ekb.eg/article_43564_42f181e3d14868981ecb8f41d0e49090.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
11
2
2019
03
01
Expression Level of Caspase-3 Gene in Colorectal Cancer Induced by Dimethylhydazine and Potential Therapeutic Role Of Mesenchymal Stem Cells and Curcumin.
123
132
EN
Heba
M.
saad Eldien
Histology and Cell Biology. COLLEGE OF Medicine. Jouf University. KSA
Amna
M.
Mostafa
Zoology Department, Faculty of Science, South Valley University, Qena
Seham
A.
Mobarak
Zoology Department, Faculty of Science, South Valley University, Qena
seham.ali@sci.svu.edu.eg
10.21608/eajbsc.2019.45825
Caspase-3 Gene is a unique protease enzyme involved in the initiation and execution of apoptosis process. The aim of this study to investigate the mRNA expressions of caspase-3 on experimentally induced colorectal cancer (CRC) in male albino rats, rats treated with adipose tissue mesenchymal stem cells (MSCs) and curcumin. Forty white male albino rats were divided into four groups 10 in each, Control group (1<sup>st</sup>): They received 0.5 ml of 25% DMSO orally once daily, while the other three groups were injected subcutaneously with dimethylhydrazine (DMH) at a dose of 20 mg/kg body weight per week for 4 consecutive weeks. After two weeks of the last DMH dose, the 2<sup>nd</sup> Group: left injected with DMH only, the 3<sup>rd</sup> group: every rat received one million cells of MSCs interperitoneally once. The 4<sup>th</sup> Group: received oral curcumin at a daily dose 100 mg/kg body weight for two weeks. The colon tissue homogenate caspase-3 mRNA expression was assayed by (real-time PCR). Caspase-3 expression level was significantly decreased in the DMH group than that of the control group while it was significantly increased in DMH+MSCs and DMH+curcumin treated groups in comparison with a DMH group. Mesenchymal stem cells and curcumin thought to have an ameliorative effect on colorectal cancer in male albino rats. Thus the development of CRC may involve changes in the mRNA level of the caspase-3 gene.
Caspase-3,Colorectal cancer (CRC),Mesenchymal Stem Cells (MSCs),Curcumin
https://eajbsc.journals.ekb.eg/article_45825.html
https://eajbsc.journals.ekb.eg/article_45825_bcdd4440de535b1e05d05b583fd93ced.pdf