Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Evaluation of Hemostaitc Parameters of Sudanese patients with liver Disease attending Khartoum Teaching Hospital
1
5
EN
Mahmoud
Mohamed
Elgari
Taibah University – college of applied medical sciences – laboratory department – Almadinah - KSA
elgari999@yahoo.com
10.21608/eajbsc.2013.16102
<strong>Background:</strong> Various hemostatic abnormalities can occur in patients with liver disease, the severity of these abnormalities is dependent on the degree of hepatic dysfunction. Liver disease can cause both quantitative and qualitative abnormalities in coagulation factors, fibrinolytic system and platelets abnormalities, with consequent variable impairment of coagulation parameters.
<strong>Methods: </strong>In this case control study we examined the 60 liver diseases patients and 30 control healthy subjects, in Khartoum Teaching Hospital (Khartoum, Sudan) during the period 10/3/2012 to 12/6/2012. Prothrombin time (PT), Activated partial Thrmboplastine time (APTT) and fibrinogen level (FL) were determined and categorized by types of liver disease, age, and sex in all study subjects.
<strong>Results: </strong>The result revealed that there were significant increased in (PT) (P=0.00), APTT (P=0.00) and significant decreased in fibrinogen level (P=0.00) among patients of liver disease when compared with control group. There were no significant changes in coagulation parameters related to age and gender.
<strong> Conclusion: </strong>There were significant increased in PT and APTT associated with liver diseases and significant decreased in fibrinogen level among patients of liver disease when compared to normal control.
Prothrombin time (PT),Activated partial Thrmboplastine time (APTT),Fibrinogen level (FL),von Willebrand Factor (vWF)
https://eajbsc.journals.ekb.eg/article_16102.html
https://eajbsc.journals.ekb.eg/article_16102_755824ea7547b0f4aa5ef10c5e460b87.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Hepatitis B and C Viruses incidence, the Risk Factors of Hepatocellular Carcinoma, is low in Aseer Region, Saudi Arabia
7
18
EN
Ali
Alshehri
Biology Department, Faculty of Science, King Khalid University, Abha, P.O Box 9004, Saudi Arabia
ali_50_50@yahoo.com
10.21608/eajbsc.2013.16103
<strong>Aim:</strong> Risks of hepatocellular carcinoma (HCC) following hepatitis B (HBV) and/or hepatitis C virus (HCV) infection are well known. The aim of this study was to determine the prevalence and risk factors of hepatitis B and C virus infections in blood donors at Aseer Region, Saudi Arabia
<strong>Methods:</strong> The study was conducted on random blood samples collected from healthy blood donor volunteers, who were referred to Blood Transfusion Centers found at Aseer region, during the period March 2012 to February 2013. All the collected blood units were screened for hepatitis B surface antigen (HBsAg), anti-HBc, HCV, human immunodeficiency virus (HIV) 1 and 2, human T-cell lymphotropic virus (HTLV) I/II, venereal disease research laboratory (VDRL) and malaria. All donated blood were checked for HBV-DNA, HCV-RNA and HIV-RNA by nucleic acid test (NAT) technology.
<strong>Results:</strong> Of 7267 (26 (0.36%) females and 7241 (99.64) males) blood donors screened, with median age of 28 (female) and 30 years (males), 71 (0.98%) were HBsAg positive of them 66 (0.91) were positive to HBV-DNA, 449 (6.18%) were anti-HBc positive of them 78 (1.07%) were positive to HBV-DNA. Cases positive to both HBsAg and HBc-Ab were 69 (0.95%) all of them were positive to HBV-DNA. There were 5 (0.069%) cases positive for HCV, none of them showed mixed infection with HVB. All positive cases for HBsAg, HBc-Ab and HCV were shown to be among male volunteers.
<strong>Conclusion</strong>: Prevalence of HCV in Asser region is very low. In the time that HBsAg is low, prevalence of HBcAb is relatively moderate. Expected incidence of heptatocellular carcinoma due to infection with HCV is much lower than HBV.
Blood donors,HBV,HBsAg,HCV,HCC,CLD,Saudi Arabia
https://eajbsc.journals.ekb.eg/article_16103.html
https://eajbsc.journals.ekb.eg/article_16103_ad12aa19ae1c2647af65719ecabbfc48.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Biochemical studies of Bacillus Thuringiensis var.kurstaki, Serratia marcescns and Teflubenzurone on cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptea: Noctuidae)
19
30
EN
El-Sheikh
T. A.
A.
Plant Prot. Res. Inst., Agric Res. Centre, Dokki, Giza, Egypt
Heba
S.
Rafea
Faculty of Agriculture, Ain-Shams- University, Egypt
El-Aasar
A.
M.
Faculty of Agriculture, Ain-Shams- University, Egypt
Ali
S.
H.
Plant Prot. Res. Inst., Agric Res. Centre, Dokki, Giza, Egypt
10.21608/eajbsc.2013.16104
Two biopesticides <em>Serratia marcescens </em>[Eubacteriales: <em>Enterobacteria</em>] (used at MC<sub>50</sub>, concentration caused 50% malformation) and <em>Bacillus thuringiensis Var. kurstaki</em> (used at LC<sub>50</sub>) and insect growth regulator Teflubenzuron (used at LC <sub>50</sub> value) were used for treatment of 2<sup>nd</sup> instar larvae of cotton leafworm, <em>Spodoptera littoralis </em>(Boisd.). Sequential combined Effect was carried out bytreating 2<sup>nd</sup> instar larvae with LC<sub>50 </sub>value of <em>B. thuringiensis </em>or Teflubenzuron then the larvae allowed to pupate on sawdust treated with<em> S. marcescens</em> at MC<sub>50 </sub> <em>.</em>The effect of these three agents were assessed by toxicity The obtained LC<sub>50 </sub>valueswere 0.113 and 165.64 ppm, for Teflubenzuron and <em>B. thuringiensis Var. kurstaki</em>. (protecto), respectively. Moreover, the activity of Chitinase, protease, trehalase, and the main components (total lipids, proteins and carbohydrates) were significantly fluctuated during the different periods of pupal stage.
Bacillus Thuringiensis var.kurstaki,Serratia marcescns and Teflubenzurone
https://eajbsc.journals.ekb.eg/article_16104.html
https://eajbsc.journals.ekb.eg/article_16104_22e13c04d3f4c9942c55178c4f498ae1.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Hyperprolactinemia as a cause of female primary infertility and its prevalence in Gezira State, Central Sudan
31
36
EN
Salah Eldin
A.
AbdElghani
Ministry of Health, Gezira State, Wad Medani, Sudan
Abdelgadir
A.
Elmugadam
-College of Medical Laboratory Science, Sudan University of Science & Technology, Sudan
-College of Applied Medical Science, Taibah University, KSA
Mohammed
Elsanousi M.4
M.
Faculty of Medicine, University of Gezira, Wad Medani, Sudan
10.21608/eajbsc.2013.16107
<strong>Background: </strong>Hyperprolactinemia is a condition of elevated serum prolactin. Prolactin is a 198-amino acid protein (23-kD) produced in the lactotrophs cells of the anterior pituitary gland. Hyperprolactinemia is the most common hypothalamo-pitiutary disorder; it can also occur secondary to use of some drugs, chronic illnesses like hypothyroidism, chronic liver and kidney disease, stress and neurogenic disorders. Idiopathic Hyperprolactinemia refers to elevation of serum prolactin in the absence of the above conditions.
<strong>Objective: </strong>The aim of this study is to explain scientifically hyperprolactinemia as a cause of female primary infertility and its prevalence in GeziraState, Central Sudan.
<strong>Method:</strong> Serum prolactin, FSH and LH levels were estimated using commercially specific enzyme immunoassay (EIA) technique in serum from 200 women (150 primary infertile and 50 fertile non-pregnant and non-lactating) attending Wad Medani teaching hospital in Wad Medani city, Central Sudan, from 2011 till 2013.
<strong>Results: </strong>Hyperprolactinemia was found in 33.3%, irregular menstrual cycle 66%, amenorrhea 6.7%, oligomenorrhoea 59.3%, normal menorrhea (33,3%) and galactorrhoea 3.3% of primary infertile women.
<strong>Conclusion: </strong>The prevalence of hyperprolactinemia was about one-third of primary infertile women in GeziraState (Central Sudan). The mainaetiology ofprimary infertility was anovulatory cycle due to high level of prolactin.
Hyperprolactinemia- female primary infertility- Gezira State
https://eajbsc.journals.ekb.eg/article_16107.html
https://eajbsc.journals.ekb.eg/article_16107_5a9d9ce3e77ee32beae2d147e44fd3b8.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Evaluation of Hematological Parameters of Sudanese Pregnant Women attending at Omdurman Al Saudi Maternity Hospital
37
42
EN
Mahmoud
Mohamed
Elgari
Taibah University – college of applied medical sciences – laboratory department – Almadinah - KSA
elgari999@yahoo.com
10.21608/eajbsc.2013.16108
<strong>Background: </strong>There are subtle and substantial changes in hematological parameters during pregnancy and the puerperium total blood volume increases by about 1.5 liter mainly to supply the needs of the new vascular bed(Taylor, 1979). Pregnant places extreme stresses on the hematological system and understanding of the physiological changes that result as obligatory in order to interpret any need for therapeutic intervention. (William and Cindy 2005). Iron deficiency is the most common cause of anemia in pregnancy (Odekule 2010). As consequences various quantitative and qualitative hematological changes occur during pregnancy including cell counts, hemoglobin levels, hematocit, leucocytes, thrombocytes, red blood cells indices, morphological changes and reticulocyte production index. (Lewis <em>et al.</em> 2001).
<strong>Methods: </strong> This a case control study in which 100 pregnant women were enrolled as study group and 50 non pregnant healthy women as control subjects. The study was conducted at Omdurman Al Saudi Maternity Hospital in period from 5/3/ 2011 to 6/6/ 2011 to evaluate hematological parameters of Sudanese pregnant and to establish classification of anemia’s on bases of complete blood count (CBC), reticulocyte production index (RPI) and peripheral blood picture.
<strong>Results: </strong>The study revealed that there were significant decreased in RBCs count, hemoglobin (Hb) and packed cell volume (PCV) of pregnant women compared to non pregnant women (P value <0.05) and significant decreased in mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) of pregnant women (P value <0.05). TWBCs count was increased significantly (P. value < 0.050) in contrast platelets count significantly lower than the normal control (P. value <0.05). On bases of blood picture we classified anemia’s of pregnancy as normocytic normochromic 37 (37%) with RPI mean value of 0.49±0.2, microcytic hypochromic 52(52%) with RPI mean value of 0.76±0.6, and dimorphic picture11 (11%) with RPI of mean value 2.1±0.8.
<strong>Conclusion: </strong>We observed significant decreased in RBC count, hemoglobin, PCV, red cell indices and platelet count during pregnancy and significant increased in leukocyte count of pregnant women when compared to non pregnant women. We found the most common type of anemia among Sudanese pregnant women is microcytic hypochromic type and likely to be of iron deficiency second class is normocytic normochromic type, and less of occurrence type is dimorphic picture types with increased reticulocyte production index results from prophylaxes iron response.
Complete blood count (CBC),Packed cell volume (PCV),Mean Corpuscular volume (MCV),Mean corpuscular hemoglobin (MCH),Mean Corpuscular hemoglobin concentration (MCHC),Reticulocyte production index (RPI)
https://eajbsc.journals.ekb.eg/article_16108.html
https://eajbsc.journals.ekb.eg/article_16108_573fc7957587b89ca5e5cf974f70d7fd.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
The application of random amplified polymorphic DNA for sandfly species identification in Saudi Arabia
43
51
EN
Reem
A.
Al-Ajmi
Zoology Department, King Saud University, Riyadh, Saudi Arabia
Mai
H.
Al-Jaser
Zoology Department, King Saud University, Riyadh, Saudi Arabia
Ahmed
A.
Al-Qahtani
-Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
-Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia
10.21608/eajbsc.2013.16109
Sandflies<br />are of great medical and economic importance as vectors of disease agents such<br />as viruses, bacteria and protozoan parasites. Because of the great importance<br />of these insects in the Kingdom, The present work has been undertaken to<br />collect and identify samples from different regions of the<br />Kingdom of Saudi Arabia, which included the Central Province (Riyadh and<br />Qassim), the East Province (El Ehsa), the West Province (AlMadinah AlMunawarh)<br />and South Province (Abha and Assir). Samples were divided into two parts: the<br />first included the head and terminalia, which were used for morphological<br />taxonomy, and the second part included the rest of the body which was used for<br />molecular taxonomy. Standard keys of morphological taxonomy were used for the<br />identification and classification of the sandflies. The collected sandflies<br />were found to belong to five species and two genera. Of these, three species<br />belonged to the genus <em>Phlebotomus, </em>these were <em>Phlebotomus</em>(<em>P.</em>)<em><br />papatasi</em>,<em> P. bergeroti</em> and <em>P.</em><br /><em> sergenti</em>. The other species belong to genus <em>Sergentomyia,</em><br />these were<em> Sergentomyia </em>(<em>S.</em>)<em> antennata</em>, <em>S. clydei</em>. <em>P. papatasi</em> was the most common<br />species in all of the collection areas (56.37%), <em>S. clydei</em><br />was the second common (23.58%) and <em>S. antennata</em> was the third common<br />species (8.4%) followed by <em>P. sergenti</em> (7.86%), then <em>P. bergeroti</em> (2.71%) . The second part<br />of each fly, including the thorax, anterior part of the abdomen and wings, were<br />used for DNA extraction. The DNA was amplified by the RAPD-PCR method using two<br />different arbitrary primers, Opa-2 and Ap-16. Species-specific banding patterns<br />were obtained by this method. Slight differences were observed in the banding<br />pattern within the species which suggested that there were individual diversity<br />or that these variations were owing to the presence of subspecies or sibling species<br />in the same species
Sandflies- DNA- Saudi Arabia
https://eajbsc.journals.ekb.eg/article_16109.html
https://eajbsc.journals.ekb.eg/article_16109_fbbd0322bfee8a16de63299fc1cc9cc9.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
The role of information sources in anemia knowledge in Qassim region, Saudia Arabia
53
57
EN
Hisham
Ali
Waggiallah
Department of medical laboratory, Faculty of medical applied science, Taibah University. P.O Box 3001, Almadenah Almonawarah, Saudia Arabia.
Mahmoud
Mohamed
Elgari
Department of medical laboratory, Faculty of medical applied science, Taibah University. P.O Box 3001, Almadenah Almonawarah, Saudia Arabia.
elgari999@yahoo.com
10.21608/eajbsc.2013.16110
Anemia is a global public health problem affecting both developing and developed countries with major consequences for human health as well as social and economic development. The aim of this study to assess the role of information sources in anemia awareness. This cross-sectional community-based study was conducted throughout the Qassim region which has been divided into 11 provinces were evaluated. A total of 1281Saudi citizens participated in this study. Each participant was asked to answer a total of 18 questions from a pre-piloted questionnaire. 1281 questionnaires were distributed the response rate was 95.7%. Males represented 62.2%. General knowledge of anemia was 89.5% of the study population. Satisfactory, fair, and poor awareness of anemia represented 20.5%, 50.5%, and 29.0% respectively. Friends and relatives were constituted the major information sources while books and journals were the lowest source. We conclude that general awareness of anemia is very good, but still more efforts should be performed through information sources to boost of anemia awareness in Saudi population in Qassim area to reach satisfactory knowledge, which reflect in life style and hygiene behavior.
information sources,Anemia,Awareness,Knowledge
https://eajbsc.journals.ekb.eg/article_16110.html
https://eajbsc.journals.ekb.eg/article_16110_d423c1251baf14ed4567dfa5c01bca65.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
The hepato-ameliorating effect of Solanum nigrum against CCl4 induced liver toxicity in Albino rats
59
66
EN
Abd-Elraheim
Ali
Elshater
Department of Zoology, Faculty of Science, South Valley University, Qena
Muhammad
Mahmud Ali
Salman
Department of Zoology, Faculty of Science, South Valley University, Qena
salman2_52014@yahoo.com
Samar
Ali
Mohamed
Department of Zoology, Faculty of Science, South Valley University, Qena
10.21608/eajbsc.2013.16111
The present study was investigated to evaluate the hepato-ameliorating and antioxidant activity of two aqueous extracts of <em>Solanum nigrum</em> (SN) against CCl<sub>4</sub>-induced toxicity in rats. Male Albino rats were divided in four groups with 8 animals in each group. Group (1) was normal group and group (2) was injected intraperitoneal (i.p.) with CCl<sub>4</sub> (1ml/kg) 3 times weekly for 2 weeks (control). Group (3) was injected with CCl<sub>4 </sub>and then treated with extract from whole plant of <em>Solanum nigrum</em> (500 mg/kg) and group (4) was injected with CCl<sub>4</sub> and then treated with extract from fruits of <em>Solanum nigrum</em> (250 mg/kg). CCl<sub>4</sub> injection enhanced activity of hepatic enzymes (AST and ALT) while it decreased serum total protein and albumin in experimental animals. It also decreased RBC, platelets count, PCV and Hb levels. However it increased WBC count. CCl<sub>4</sub> injection increased level of lipid peroxidation resulting in a decrease in the level of enzymatic and non enzymatic antioxidants. Treatment with two extracts of <em>Solanum nigrum</em> altered these changes to near normal levels. Buthepato-ameliorating and antioxidant effects of extract of <em>Solanum nigrum</em> fruits were found to be better than those of extract from whole plant of <em>Solanum nigrum</em>.
Carbon tetrachloride,Solanum nigrum,liver toxicity,albino rats,fruits of Solanum nigrum,Antioxidant enzymes
https://eajbsc.journals.ekb.eg/article_16111.html
https://eajbsc.journals.ekb.eg/article_16111_a88e92462309b3eac1933195565c0228.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Effect of lead on some haematological and biochemical characteristics of Clarias gariepinus dietary supplemented with lycopene and vitamin E
67
89
EN
Usama
M.
Mahmoud
Zoology Department, Faculty of Science, Assiut University
Abdel-
M.
Ebied
Zoology Department, Faculty of Science, South Valley University
Salwa
M.
Mohamed
Zoology Department, Faculty of Science, South Valley University
10.21608/eajbsc.2013.16112
The present study aimed to investigate the potential protective effects of lycopene (9 mg/kg ) in comparison with vitamin E (50 mg/kg) against the impacts of lead (Pb) toxicity(7 mg/l: 1/4 of 96 h LC50) on fishes Pb exposed for 15 and 30 days. Pb impacts were evaluated in terms of haematological and biochemical characteristics. The activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly increased due to Pb. Treatment with Pb caused a significant increase in mean corpuscular haemoglobin concentration, serum glucose, total lipid,urea and creatinine and Lipid peroxidation in liver,kidney and gills tissues. On the other hand, Pb significantly caused decline in serum total protein,albumin, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume and mean corpuscular haemoglobin . It was observed that supplementation of lycopene and vitamin E decreases the toxic effect of lead.
Fishes,Lead,lycopene,Vitamin E,haematological,biochemical
https://eajbsc.journals.ekb.eg/article_16112.html
https://eajbsc.journals.ekb.eg/article_16112_a902470979045968ce80df34814c327c.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Haemolymph of Tuta absoluta: Collection procedure and chemical characteristics
91
95
EN
Tarek
R.
Amin
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
aziza
H.
Mohamady
Central Agricultural Pesticides Laboratory, Agricultural Research Center, Dokki, Giza, Egypt
10.21608/eajbsc.2013.16113
A technique used to collect haemolymph of the tomato leafminer; <em>Tuta absoluta</em> (Lepidoptera) fourth larval instar was described. It depends on centrifugation of the larvae at a given speed. This technique has the advantage that it enables collecting blood from <em>T. absoluta</em> larvae whereas manual expression of blood from the cut ends of prologs is impossible. Approximatry 11 µl of haemolymph from 8-10 larvae were collected after centrifugation for 10 min at 1500 r.p.m.. The chemical characteristics of haemolymph plasma collected were studied. The haemolymph of the tomato leafminer contains 29.27, 390, 79, 440, 62.5 and 137.5 µg / ml of chloride, phosphorus, calcium, magnesium, sodium and potassium, respectively. Na: k ratio was 0.45, indicating the presence of low sodium index as an advanced feature like other Lepidopterans. The measured high magnesium content, and alkaline pH (=7.3) might be due to <em>T. absoluta </em>food habit as aphytophagous insect.In general, the main characteristics of the tomato lefminer are more or less similar to that of other species of Lepidoptera. The centrifugation technique is an efficient method to collect blood from small insects, but the optimal velocity of centrifugation must be detected for each species.
Haemolymph,collection,ions,Tomato leafminer,Tuta. absoluta
https://eajbsc.journals.ekb.eg/article_16113.html
https://eajbsc.journals.ekb.eg/article_16113_54689d8e5d2dbaa9f941e795a302cff5.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Enzyme Inhibition (AChE) in Brain of Oreochromis mossambicus due to Pesticidal Pollution of Herbicide “Pursuit”
97
100
EN
Farhina
Pasha
Department of Biology, Girls Science Section, University of Tabuk, Kingdom of Saudi Arabia
10.21608/eajbsc.2013.16114
In the present investigation, the effect of three sublethal concentrations of Pursuit, that is, 63.7 ppm, 85 ppm, and 127.5 ppm in <em>Oreochromis</em> <em>mossambicus</em> was studied. Pursuit inhibited acetyl cholinesterase in the brain of <em>Oreochromis</em> <em>mossambicus</em> by increasing the K<sub>m</sub> and V<sub>max</sub>, thereby acting as a mixed inhibitor. The assay of brain AChE is thus useful for monitoring pesticide toxicity of fish.
Acetyl Cholinesterase,Oreochromis mossambicus,Pursuit and Mixed inhibitor
https://eajbsc.journals.ekb.eg/article_16114.html
https://eajbsc.journals.ekb.eg/article_16114_004c8d59004ac543d06e2ebc749d70ff.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Screening for Cervical Cancer and Its Association with Human Papilloma Virus (HPV) among Sudanese Women
101
106
EN
Moneira
A.
Mansour
Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Al Madenah, Al Monawarah, KSA
Magdi
M.
Salih
Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan
Ahmed
E.
Shomo
Department of Pathology, Faculty of Medicine and Health Sciences, International University of Africa, Khartoum, Sudan.
Amel
O.
Bakheit
College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum, Sudan.
Mogahid
M.
Elhassan
College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan
10.21608/eajbsc.2013.16115
This study investigates the presence of human papilloma virus (HPV) on cervical smear among women in KhartoumState. Four hundred specimens were taken from patients who attended different hospitals in KhartoumState during a period from July 2008 to July 2009. The specimens were processed and screened using cytological technique (Papanicolaou stain) and PCR for HPV detection.
The pre-cancer cells were detected in 30/400 (7.5%) specimens among which cervical glandular intraepithelial neoplasia (CGIN) was found in one cases 1/30 (0.3%), the details were as follows: mild dyskaryosis was present in 18/30 (4.5%), moderate dyskaryosis in 5/30 (1.3%), and severe dyskaryosis 6/30 (1.5%). On the other hand, screening for HPV among the enrolled subjects revealed high ratio (36.0%). HPV was detected in all cases that had cytological changes except one (29 out of 30 cases (96.7%)). Infections other than HPV were observed during cytological assessment which include <em>T. vaginalis</em> 8 (2.0%), <em>Candida</em> spp. 11(2.8%), and <em>Actinomyces</em> spp. 5 (1.3%).
The study concluded that the prevalence of HPV infection is high in Sudanese women (36%) who were revealed as 96.6% in pre-cancer cases and 83.3% in cervical cancer patients.
Cervical Cancer,human papilloma virus (HPV),Sudanese women
https://eajbsc.journals.ekb.eg/article_16115.html
https://eajbsc.journals.ekb.eg/article_16115_21bfbe8b52c7a9ecf0578aae00a835df.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Lipoprotein (a) level and other laboratory variables in diabetic patients
107
112
EN
Ahmed
Mohammed
Ahmed
Department of Medical Laboratories - College of Applied Medical Sciences - Taibah University - Almadinah Almunawara - Kingdom of Saudi Arabia.
Hisham
Ali
Waggiallah
Department of Medical Laboratories - College of Applied Medical Sciences - Taibah University - Almadinah Almunawara - Kingdom of Saudi Arabia.
Mahmoud
Mohamed
El-gari.
Department of Medical Laboratories - College of Applied Medical Sciences - Taibah University - Almadinah Almunawara - Kingdom of Saudi Arabia.
10.21608/eajbsc.2013.16116
<strong>Objective:</strong> to evaluate the clinical and diagnostic significant of lp (a) levels and other lipid and apolipoprotein profiles in diabetic patients compared with different categorization of diabetic patients.
<strong>Method:</strong> Case control study determined the levels of lp (a), apolipoprotein A1, apolipoprotein B, Total Cholesterol (TC), Triglyceride (TG), Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL) and fibrinogen in 100 diabetic patients with both types (40 were Insulin Dependent Diabetic Mellitus, IDDM and 60 were Non-Insulin Dependent Diabetic Mellitus NIDDM) and compared with normal healthy subjects.
<strong>Results:</strong> lp (a), apolipoprotein B, TC, TG and LDL were significantly higher in patients than control healthy subjects (p<0.05), apolipoprotein A1 and HDL were significantly lower in patients than control healthy subjects (p<0.05). No significant difference found in Fibrinogen between patients and controls. Lp (a) levels in obese patients significantly higher than in normal weight (p<<0.05). lp (a) in patients with Coronary Heart Disease (CHD) was significantly higher than patients without CHD (p<0.05). Lp (a) was significantly higher in NIDDM than in IDDM (p<0.05).
<strong>Conclusion:</strong> The study confirmed that the Lp (a) and other lipid and apolipoprotein parameters were significantly raised (good lipid decreased (apo A1 and HDL)) in diabetic patients compared to healthy subjects, and lp (a) are important diagnostic marker that can be used for early detection of atherosclerotic cardiovascular disease (CVD) in diabetic patients.
Lipoprotein (a),Apolipoprotein A1,Apolipoprotein B,CHD,IDDM and NIDDM
https://eajbsc.journals.ekb.eg/article_16116.html
https://eajbsc.journals.ekb.eg/article_16116_fec9210d8309467d82c00458057d6eb6.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Impact of thermal stress on the haemolymphal proteins, biological and economical characters of the silkworm, bombyx mori L.
113
122
EN
Rehab
Hosny
Taha
Sericulture Research Department (SRD), Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Egypt.
10.21608/eajbsc.2013.16117
The purpose of this study is to determine the impact of different thermal stresses on univoltine <em>Bombyx mori</em> embryos and grown larva in the terms of larval haemolymph proteins , biological and economical characters. The experiment was designed to expose egg groups to different temperatures as follows; a group exposed to 0°C for one hour, a group exposed to 0°C for two hours, a group exposed to 40°C for one hour and a group exposed to 40°C for two hours, followed by 2 hours recovery at room temperature in addition to a control group, the same was applied to the grown larval groups. The larval haemolymph of tested groups were subjected to protein analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Data revealed that considerable alteration in temperature hamper the protein activities which affects the embryonic development and hatching of eggs. 40ºC for two hours was lethal dose for embryo. The highest measured parameters were recorded for eggs exposed to 40ºC for one hour. While for grown larvae, the highest results were recorded for larvae exposed to 0ºC for 1 hour. Thermal stresses induced protein changes with the appearance of new bands. The epigenetic degree of similarity recorded 50% between the control egg group and the egg groups exposed to 0ºC for 2 hrs and 40ºC for 1 hrs. as well as, for grown larva, between control larval group and larval group exposed to 40ºC for 2 hrs.
Bombyx mori,egg,larva,thermal stress,haemolymphal proteins,biological,economical characters
https://eajbsc.journals.ekb.eg/article_16117.html
https://eajbsc.journals.ekb.eg/article_16117_f0d9e8f010644b69f50fb6e98c844775.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
2090-0767
2090-083X
5
1
2013
06
01
Ribosomal DNA sequences reveal a parasitic septate gregarine (Apicomplexa: Gregarinia) in the scorpion Leiurus quinquestriatus in Egypt
123
129
EN
Moustafa
M. H.
Sarhan
Department of Zoology, Faculty of Science, Al-Azhar University, Assiut
10.21608/eajbsc.2013.16118
Ribosomal RNA genes are ubiquitously present and nucleotide sequences of many regions are relatively conserved among many organisms. Many specific PCR primers have been developed for amplification of the rDNA fragments from particular taxa. However, many authors use universal primers which can bind to conserved regions to detect protozoa pathogens that are obligate parasites of digestive tracts and body cavities in the analysed DNA sample of invertebrate animals. In this study, uncultured gregarine clones were detected by PCR and rDNA sequencing in the scorpion, <em>Leiurus quinquestriatus</em> (Scorpiones: Buthidae). Comparison of the amplified rDNA fragments with sequences deposited in GenBank revealed that analysed sequences corresponded to 18S rDNA from the genus Gregarina (88 % identity with <em>Xiphocephalus ellisi</em>). To our knowledge, this is the first record of scorpion infected with gregarines.
rDNA,Leiurus quinquestriatus,Scorpion,Apicomplexa,gregarines
https://eajbsc.journals.ekb.eg/article_16118.html
https://eajbsc.journals.ekb.eg/article_16118_33fc88f31fec67b0b44733c9fcdd34aa.pdf