Prolificacy Detection in Egyptian Sheep using RFLP-Specific PCR
Abulyazid
I.
Atomic Energy Authority
author
Abdalla
S.
Biochemistry-Faculty of Science Hellwan Uni.
author
Sharada
M.
Biochemistry-Faculty of Science Hellwan Uni.
author
Saleh
M.
Atomic Energy Authority
author
Hassanin
F.
Atomic Energy Authority
author
text
article
2011
eng
Booroola gene (FecB) was the first major gene for prolificacy identified in sheep. In this study twenty two (11 twin producing female, 7 single lamb producing female, and 4 male) crossbred sheep were tested for the presence of the FecB mutation of BMPR1B. The females were selected for their twin production in three repetitive production cycles while the males were selected for being produced from prolific females as above. Forced restriction PCR of the FecB gene, 190 base pair (bp) was amplified using specific primer designed to introduce a point mutation in the resulting PCR products with FecB carrier sheep containing an AvaII restriction site (G|GACC). The FecB DNA test showed that there were no carriers for the FecB mutation in the selected prolific sheep sample.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
1
4
https://eajbsc.journals.ekb.eg/article_16139_952a11cf7722ffdce2dccae3c0dc0411.pdf
dx.doi.org/10.21608/eajbsc.2011.16139
Physiological studies on the effect of a bradykinin potentiating factor (BPF) isolated from scorpion venom on the burnt skin of alloxan-induced diabetic Guinea pigs.
Abd-Elraheim
Elshater
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
author
Muhammad
Salman
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
author
Asmaa
Abd-Elhady
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
author
text
article
2011
eng
It is well known that, Wound healing in the diabetes is enormous, growing problem and having cost Medicaid. Therefore, this study produces an exciting prospect, to improve diabetic burn healing, and evaluates the ability of a bradykinin potentiating factor (BPF) isolated from scorpion venom (Buthus occitanus) in treatment of burns in diabetic male Guinea pigs. Male Guinea pigs of approximate (550 g body weight each) were divided into five groups. In the normal group; Guinea pigs were interaperitoneally (i.p) injected with 100 µL saline solution. The second, served as control group which were injected with 100 µL saline solution then standard burns were obtained on the dorsal skin. The 3rd, 4th and 5th groups were i.p. injected with (750 mg /kg, body weight) with a single dose of Alloxan, then standard burns were obtained, after that the 4th group was treated by BPF in 100 µL saline solutions (1μg/gm. b. w.) topically and the 5th group treated with BPF (1μg/gm. b. w.) topically and interperitonialy (1μg/gm. b. w. twice a week). Every group contains 20 animals and sacrificed at 15 and 30 days post-treatment by BPF (10 animals per each). The results are recorded after monitoring the CBC including (RBCs, WBCs, platelets, Hb content and HCT percentage), lipid profile including (total cholesterol (TC), triglyceride (TGs), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL)) and the skin antioxidant status (catalase activity (CAT) and superoxide dismutase (SOD) and serum nitric oxide (NO) beside malondialdehyde (MDA) concentration were also monitored during the study compared to normal animals. Also serum glucose level was monitored. The recorded results declared that, the treatment with BPF has shown an ameliorative effect on burn healing in diabetic animals. These observations and investigations were the pacemaker for the hypothesized ameliorating activity of BPF in the present study.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
5
15
https://eajbsc.journals.ekb.eg/article_16140_dad829faf99db443d5ab065d2f13360b.pdf
dx.doi.org/10.21608/eajbsc.2011.16140
Toxic effects of mancozeb containing formulations and neemix pesticides on kidney function and ultrastructure of albino rats
Mahmoud
Salem
Zoology department, Faculty of science, Al-Azhar University, Nasr city, Cairo, Egypt
author
text
article
2011
eng
Chronic effects of mancozeb contaning formulations (Anadol, Blanko, Deltathin, Tazolin) and Nemic pesticides on kidney function and ultrastructure of albino rats were studied. Thirty six male albino rats weighing 200-220 g were housed in the laboratory under natural condition and received normal commercial ration and water ad-lib then subdivided into six groups, six animals each: control, Anadol, Blanko, Deltathin, Nemic and Tazolin groups. Animals of each pesticide groups were received 25% of the LD50 of the corresponding pesticide once weekly for eight weeks using stomach tube.
Results revealed significant decrease (p < 0.05) in serum total protein and albumen of all treated groups compared to control group. The lowest value of total protein (6.91 + 0.51 g / dl) and albumen (3.86 + 0.12 g / dl) was recorded for Tazolin group. In the other hand serum urea and uric acid were significantly increased (P < 0.05) in all treated groups compared to control group. Tazolin group comes on the top record (42.34 + 5.65 mg / dl for urea and 8.44 + 1.02 mg / dl for uric acid).
A significantly elevated serum urea and uric acid concentrations accompanied with decreased total protein and albumen levels usually indicates impaired glomerular function.
Results of the kidney ultrastructure of the different treated groups shows marked alterations compared to control group. Renal glomerulus, shows, degenerated basal lamina, endothelial cells, podocytes and degraded podocyte pedicels. Proximal convoluted tubule with fragmented brush border, plasmalemma, large number of vacuoles, dense granulation, mitochondria and lysosomes are seen specially in Tazolin treated group. Distal convoluted tubule with, plasmalemma, large number of vacuoles, dense granulation, mitochondria and lysosomes and secreted granules was also seen in all treated groups. It is concluded that the contamination produced as the result of using mancozeb containing pesticide and Nemic pesticide for long time may have severe toxic effect on kidney function and ultrastructure.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
17
30
https://eajbsc.journals.ekb.eg/article_16141_83be2ba3271ba021951d564b0240413e.pdf
dx.doi.org/10.21608/eajbsc.2011.16141
The role of Interferon- γ Receptor-1 Gene (-56 T < C) polymorphism in development of susceptibility to pulmonary Tuberculosis in Central Sudan
Attalla
Attalla
Institute of Molecular Medicine, University of Gezira, Wad Medani, Sudan
author
Mogahid
Elhassan
College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan
author
Nagla
Mohammed
Institute of Molecular Medicine, University of Gezira, Wad Medani, Sudan
author
Miskelyemen
Elmekki
College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan
author
Adil
Mirghani
Institute of Molecular Medicine, University of Gezira, Wad Medani, Sudan
author
text
article
2011
eng
Background: It is estimated that every year 8 million new cases of tuberculosis occur globally and that 2 to 3 million people die annually from the disease. Human genetic variation is an important determinant of the outcome of infection with Mycobacterium tuberculosis. The aim of this study is to determine genetic susceptibility to tuberculosis among patients and their control by identifying the frequency of some IFNγR1 polymorphisms in development of susceptibility to pulmonary tuberculosis in Gezira state of Central Sudan.
Methods: Polymorphism (-56 T< C) was analyzed in both 126 confirmed positive TB cases and 200 matched controls using PCR-RFLP method.
Results: The results showed that the distribution of IFNγR1-56 T < C genotype differs significantly between tuberculosis patients and controls and those carrying the mutant C allele were associated with two times reduced risk for susceptibility to Tuberculosis (P-value = 0.017, odds ratio = 1.6, 95% CI = 1.025 – 1.441), that mean the mutation IFNγR1 -56 T < C gives protection two times more than the wild type allele. However,more studies in IFNγR1 gene, and polymorphisms in IFNγ gene, needs to be performed with larger sample size
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
31
35
https://eajbsc.journals.ekb.eg/article_16142_c736bfdf63cbfad19aaea58642760009.pdf
dx.doi.org/10.21608/eajbsc.2011.16142
Proteolytic digestion of blood meal in Ornithodoros erraticus, a vector of Borrelia crocidurae causing relapsing fever in Egypt.
Nawal
Shanbaky
Department of Entomology, Faculty of Science, Ain- Shams University
author
Nadia
Helmy
Department of Entomology, Faculty of Science, Ain- Shams University
author
Hala
Khater
Research and Training Center on Vectors of Diseases, Ain- Shams University
author
Ayat
Yousery
Department of Entomology, Faculty of Science, Ain- Shams University
author
text
article
2011
eng
Proteolytic activity was demonstrated in each of the midgut lumen content (MLC) and midgut wall (MW) of the mated unfed and fed female and male Ornithodoros erraticus. In both midgut compartments proteinase activity levels increased after feeding to reach at their peaks about double times the levels in the unfed controls. The peak levels of the proteolytic activity were attained on 1 and 1 – 2 days after feeding (daf) in the MLC (48 and 31.10 – 32.63 µg alanine ×10³/ min/ mg gut content) and on 5 – 6 and 5 – 7 daf in the MW (196.33 – 200.33 and 156.80 – 161.23 µg alanine ×10³/ min/ mg tissue) in the female and male tick, respectively. However, the proteolytic activity in the MLC was much lower than that in the MW of all the corresponding physiological states studied, and represented about one fourth and one fifth of it at the peak activity levels in the female and male tick, respectively. Generally, proteolytic activity was higher in the female than male ticks in the unfed and up to the 2nd daf in the MLC and in all the corresponding examined states in the MW throughout the period of study (20 daf). Changes in the level of proteolytic activity in each midgut compartment of the female and male ticks infected with Borrelia crocidurae followed almost the same pattern in the uninfected controls. However, the level was lower in the MW of Borrelia infected ticks than uninfected ones throughout most (2–20 daf) of the period of study. The present results were discussed and conclusions conformed to the view of intracellular proteolytic digestion of blood meal in the MW, but did not exclude a possible role of extracellular proteolytic digestion in the MLC.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
37
50
https://eajbsc.journals.ekb.eg/article_16143_d76af217f087676445553a478e6c844a.pdf
dx.doi.org/10.21608/eajbsc.2011.16143
Impact of Spinetoram on Some Nitrogenous Components Related to Protein Metabolites in the Cotton Leaf Worm, Spodoptera littoralis (Biosd.)
Tarek
Amin
Plant Protect. Res. Instit, Agric. Res. Center, Dokki, Giza, Egypt
author
Nedal
Fahmy
Plant Protect. Res. Instit, Agric. Res. Center, Dokki, Giza, Egypt
author
text
article
2011
eng
A laboratory study on the 4th instarlarvae of cotton leaf worm. , Spodoptera littoralis (Biosd.) was carriedout to detect and quantify some nitrogenous components related to proteinmetabolism and also to evaluate the secondary effect of the bioinsecticide;Spinetoram. It is the first time to detect albumin and creatinine in thisspecies. Acute and latent effects using three concentrations revealed that theexcretory product; uric acid was significantly reduced 6-days post treatment bythe highest concentration (6.67ppm). It was 14.15 and 10.94 ug/mg protein forcontrol and treated larvae, respectively. Creatininewhich is an end product of phosphocreatine that acts as energy storage inskeletal muscles and other tissues is significantly increased on the 4thday post larval treatment. The insecticidal concentration range was notcritical in most cases. Albumin was significantly reduced at the 4thday post treatment and this reduction extended significantly to 6thday for all concentrations.Glutamic oxaloacetic transaminase (GOT) and glutamicpyruvic transaminases (GPT) titres were significantly reduced as compared tocontrol at the same time, the situation that reflects the importance ofSpinetoram latent effect. Spinetoramcould affect protein metabolism of the cotton leaf worm for energy reserves,metabolic enzymes, protein quality and excretory metabolism point of view
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
51
64
https://eajbsc.journals.ekb.eg/article_16144_ad760662c2e48279ebcedb857f77f39f.pdf
dx.doi.org/10.21608/eajbsc.2011.16144
Evolutionary Genetic Analysis of the cytochrome b gene variation in the Salmo trutta fario with other salmons
Abolhasan
Rezaei
Department of Genetics-School of basic science, Islamic Azad University Tonekabon Branch,Iran
author
Sheyda
Akhshabi
Young Researchers Club, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
author
text
article
2011
eng
PCR amplification and direct sequencing of regions of the cytochrome b mitochondrial genes was carried out on Iranian populations of the salmo trutta fario species. Samples of salmo trutta fario were also examined and used as an out group in the phylogenetic analysis. Results based on 1192 bp indicated differentiation between fario morphs from Iran and other salmonids also Iran and other country had collected. Despite the large phenotypic differences within salmonids, very low genetic variation was found. On the basis of the cytochrome b sequences studied, Salmo carpio and Salmo fibreni, which have been described as good species, there were high homology between salmo trutta fario , salmo trutta and salmo trutta caspius (99%), but regards salmo salar the rate of homology 93%. Regards salmo trutta fario, in hatchery trout has not red colour strips time of hatchery in salmo trutta fario whereas has a bluish grey body colour, However, they are larger than Atlantic salmon, salmo trutta caspius and salmo trutta, more regular in shape, and less intensively pigmented, moreover there are red spots are always observed in populations, however, the shape and size of salmo trutta fario is different with salmo trutta caspius and salmo trutta but the among of homology were high (99%). The salmo trutta fario and salmo trutta caspius are living in the Rivers of North of Iran that connected to Caspian Sea; perhaps these species with themselves has been conjugated, and regards salmo salar is living in the Atlantic Ocean and other Sea and Rivers connected to it, that so far to salmo trutta fario populations in Iran. However we cannot report exactly that how much homology between sequences, because already there are not complete sequence in GeneBank regards salmo trutta caspius and salmo trutta fario.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
65
71
https://eajbsc.journals.ekb.eg/article_16145_e5d0cdc969bcd97da0210b1df455f372.pdf
dx.doi.org/10.21608/eajbsc.2011.16145
Variation in esterases activity of different races and hybrids of Bombyx mori l. as a tool for genetic relationships analysis
Souad
Mahmoud
Sericulture Research Department- Plant Protection Research Institute, Egypt
author
Saadya
El-Bermawy
Department of Biology and Geology, Faculty of Education, Ain Shams University, Egypt
author
Rehab
Taha
Sericulture Research Department- Plant Protection Research Institute, Egypt
author
text
article
2011
eng
Variation in esterases activity in different silkworm races and hybrids were investigated to determine the usefulness of this marker for the assessment of genetic variation among them. A study on the haemolymph esterases pattern of different silkworm populations from different origins (two races and five hybrids) kept in Egypt was undertaken for enhancing the breeding program in the Sericulture Research Department (SRD).
The larval haemolymph showed esterolytic activity capable of hydrolysing α and β naphthyl acetate substrates. A total of 14 α and 10 β – esterase and 21 α and 24 β-esterase bands were detected during the fourth and the fifth larval instars, respectively.
By evaluating the degree of similarity among the tested populations, it was found that similarity of α esterase between Race 2, Hybrids 2 and 3 during the fourth larval instar was 50%. The similarity percentage was 40% between Race 2 and Hybrid 5 during the fifth larval instar. For β esterase, the similarity was 66% between Race 2 and both Hybrid 1 and Hybrid 4 during the fourth larval instar and was 66% between Race 2 and Hybrid 5 during the fifth larval instar.
It may be recommended to dispense Race 2 from the breeding program as it shows the highest degree of similarity with the other tested populations.
At the physiological level, esterases appeared to be a suitable marker to estimate the genetic variation among Bombyx mori races and hybrids.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Egyptian Society of Biological Sciences
2090-0767
3
v.
1
no.
2011
73
82
https://eajbsc.journals.ekb.eg/article_16146_450a0c860b59d63d5760103d1794983f.pdf
dx.doi.org/10.21608/eajbsc.2011.16146