Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea

Mar&#039;ie, Zeinab; Allam, Mohammad

doi:10.21608/eajbsc.2019.63943

Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea

Document Type : Original Article

Authors

Zeinab Mar'ie ¹
Mohammad Allam ²

¹ 1- Zoology Department, Faculty of Science, South Valley University, Egypt 2- Science Departments, Faculty of Education, Hurghada , South Valley University, Egypt

² Zoology Department, Faculty of Science, South Valley University, Egypt

10.21608/eajbsc.2019.63943

Abstract

The report compared six parrotfish species (Scarus ferrugineus, Scarus psittacus, Scarus frenatus, Chlorurus sordidus, scarus genazotus and Cetoscarus bicolor) for molecular diversity and phylogenetic using eleven ISSR primers, the obtained results showed a total of 235 DNA fragments; 11 were common bands, 65 were specific bands and 159 were polymorphic bands. Additionally, the results indicated the largest similarity degree 58 between (Chlorurus sordidus and scarus genazotus), while the lowest similarity degree 35 was found between (Scarus frenatus and Chlorurus sordidus). The results that occurred from ISSR analysis found to be more reliable for differentiating species of fishes particularly parrotfish.

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