Effect of Selenium and Bee Pollen Against Immunotoxicity and Hepatotoxicity Induced by Cadmium in Male Albino Rats.

Cadmium is a toxic naturally occurring element that affects numerous organ systems in humans, the present study was designed to study the role of bee pollen and selenium against cadmium chloride induced immunotoxicity and Hepatotoxicity in male albino rats.
       Forty (40) male albino rats were used for the experiment. Group I was intraperitoneal (i.p.) Injected with saline solution, G2 (i.p.) Injected with CdCl2 (1 mg/kg b.w.), G3 were (i.p.) Injected with CdCl2 (1 mg/kg b.w.) and then receive selenium (i.p.) Injection at a dose of (0.5 mg/kg/day). G4 (i.p.) Injected with CdCl2 (1 mg/kg b.w. day by day) and then received bee pollen orally at a dose of (10 g/kg/day). Animals were exposed to treatment once daily for 10weeks. After the last day of treatment, animals were sacrificed and blood samples collected for hematological analysis and serum separated for serum liver enzymes analysis and the liver separated for oxidative stress analysis. Results obtained in this present study revealed a significant increased at (p>0.05) in WBCs, Monocyte , Lymphocyte , Granulocyte and The activities of IgG and IgM, aspartate aminotransferase (AST), alanine transferase (ALT) ,alkaline phosphatase (ALP), (Total- Direct) bilirubin and Malondialdehyde (MAD), while showed a significant decreased at (p>0.05) in albumin and protein, Glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT) in group treated with CdCl2 only when compared with normal group. However, Effects of bee pollen and selenium  on immunotoxicity and hepatic injury due to cadmium-induced oxidative stress  revealed a significant decreased at (p>0.05) in WBCs, Monocyte, Granulocyte and The activities of IgG  except Lymphocyte and IgM are not affected also aspartate aminotransferase(AST), alanine transferase (ALT) ,alkaline phosphatase (ALP), (Total- Direct) bilirubin and Malondialdehyde (MAD) showed a significant decreased at (p>0.05), while Total protein, Albumin, Glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT) showed a significant increased at (p>0.05)  when compared with control group. It is concluded that the bee pollen and selenium have antioxidant properties