Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.

Aly, Sara; Saad, Mohamed E.; Hendam, Ashraf; Abdel-Hamied, Amina; Al-Sadek, Ahmed Farouk; Madboly, E. A.; Barakat, Hoda S.; El-Atroush, H.

doi:10.21608/eajbsc.2022.272232

Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.

Document Type : Original Article

Authors

¹ Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt.

² Department of Nucleic acid, proteomics and Genomics, Genomics facility central lab, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center ARC, Egypt. - Department of Biology, Faculty of Science, Taibah University, Almadinah Almonawarah, Kingdom of Saudi Arabia.

³ Bioinformatics Unit, Central Lab for Agricultural Experts Systems, Agricultural Research Center (ARC), Giza, Egypt.

⁴ Department of Nucleic acid, proteomics and Genomics, Genomics facility central lab, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center ARC, Egypt.

⁵ The Central Laboratory for Date Palm Research and Development (CLDPRD), Agricultural Research Center (ARC), Giza, Egypt.

10.21608/eajbsc.2022.272232

Abstract

Date palm (Phoenix dactylifera L.) is related to the family Arecaceae, which is considered one of the most ancient economically cultivated crops. Mainly, it is grown in the arid regions of the Middle East and North Africa. The crucial matter in maintaining the diverse number of date palm cultivars in Egypt is its biodiversity conservation of it. In order to progress programs and cultivar characterization and conservation to combat genetic erosion, we must estimate the genetic variability and right date palm cultivar identification this is the important point to present a comprehensive investigation for Egyptian date palm genome variations and develop novel DNA markers (SNPs and indels) in four date palm cultivars using SOLiD sequencing.

Keywords