Modulation of autophagy enhances the apoptotic cell death in human T lymphoma cells treated with Anthocyanin

El-Sokkary, Gamal H.; Sayed, Douaa M.; Fuad, Salwa A.; Abdel Shakor, Abo Bakr M.

doi:10.21608/eajbsc.2013.16098

Modulation of autophagy enhances the apoptotic cell death in human T lymphoma cells treated with Anthocyanin

Document Type : Original Article

Authors

¹ Department of Zoology, faculty of Science, Assiut University, Assiut, 71516, Egypt

² Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, 71516, Egypt.

10.21608/eajbsc.2013.16098

Abstract

In the present study, the effect of modulation of autophagy induced by
anthocyanin on cell death of Human T lymphoma cells (Jurkat) was studied.
Anthocyanin was abstracted from dry petals of Hibiscus sabdariffa,
aquous solution of anthocyanin was added to cells at different concentrations
then cell viability was determined by trypan blue exclusion method. Autophagy
induced by anthocyanin was inhibited by 5 mM NH₄CL which halts
lysosomal enzymes and accordingly preventing autolysosomes formation. On the
other hand, Autophagy was enhanced by glucose starvation. In both experiments
of autophagy inhibition and enhancement, cell viability was studied to
investigate the effect of autophagy modulation on cell viability. The results
of this work revealed that both inhibition and enhancement of autophagy induced
by anthocyanin lead to massive cell death. Immuno-detection of active caspase
3, one of the major hallmark of apoptotic cell death revealed remarkable
increase of active caspse3 upon autophagy inhibition or enhancement. In
conclusion, modulation of autophagy induced by anthocyanin lead to increasing
of apoptotic cell death

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