Purification of perforin and study expression of perforin in leukemia patient

Ahmed, Najwa Sh.; Fayyad, Amal G.S.; Mohammadi, Salimeh; Hamza, Subhi Jawad; Al-Faisal, Abdul

doi:10.21608/eajbsc.2013.16090

Purification of perforin and study expression of perforin in leukemia patient

Document Type : Original Article

Authors

¹ Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq.

² University Kebangsaan Malaysia, Faculty of Sci. and Technology, School of Bioscience

³ Baghdad University, Science college, Biotechnology

⁴ Genetic engineering and biotechnology institute, Baghdad University

10.21608/eajbsc.2013.16090

Abstract

This work aimed to study compare of extraction, purification and study gene expression of perforin from ALL, CLL and healthy, to chive this goal, blood samples were collected from 10 Leukemia cases (5 ALL and 5 CLL) and 5 healthy. Comparison of the molecular weight of purified perforin from these groups shows no detectable differences as compared with that of standard perforin which specified as 70KDa using SDS-PAGE. However, gene expression by using western blotting, it was found that the level of perforin in these groups were different due to differential gene expression between patients, and healthy control. Hence high levels were found in healthy individuals and CLL patient as compared with ALL patients as expressed by the presence of intense band.

Keywords